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Archive > Volume 2 Issue 1 > 2010,2(1):41-47. DOI:10.3969/j.issn.1674-6384.2010.01.004 Prev Next

Original articles

Transformation of Compound K from Saponins in Leaves of Panax notoginseng by Immobilized β-Glucanase

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    Abstract:
    Objective To prepare an active anti-tumor component, compound K (C-K), from saponins in leaves of Panax notoginseng (SLPN) using immobilized β-glucanase. Methods Two entrapments, alginate gel-1 (Alg 1) and alginate gel-2 (Alg 2), were evaluated for their ability to immobilize β-glucanase. The amount and purity of C-K obtained from the transformation process were analyzed by HPLC, and the immobilizing parameters were optimized. Results β-Glucanase can be immobilized and reused with either of the entrapment. However, using Alg 1 resulted in higher enzyme activity than Alg 2. The optimal concentration of the immobilized enzyme was 10%; The optimal crosslinking time was 4–6 h; and the optimal concentration of the crosslinking agent was 6%–7%. Conclusion Immobilized β-glucanase shows sustained enzyme activity, good ethanol tolerance, and was reusable for the preparation of C-K from SLPN.

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