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Archive > Volume 3 Issue 1 > 2011,3(1):75-78. DOI:10.3969/j.issn.1674-6384.2011.01.016 Prev Next

Original articles

Smashing Tissue Extraction and GC Analysis of Active Fatty Acids from Oil Cake of Perilla Seeds

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    Abstract:
    Objective To optimize the extraction technology of perilla seeds oil from the oil cake of perilla seeds (OCPS) by using the contents of active fatty acids as evaluation standard. Methods The fatty acids were extracted from OCPS, the residue of perilla seeds after cold-press, by smashing tissue extraction (STE), the new technology selected through comparing with classical leaching extraction (LE), Soxhlet extraction (SE), ultrasonic extraction (UE), and supercritical-CO2 fluid extraction (SFE). For optimized condition of STE, orthogonal test was designed and completed. The contents of five fatty acids in extracted oil and OCPS were determined by GC. Results The optimized extraction parameters were smashing for 1.5 min under extraction power of 150 W and 1:6 of the material/solvent ratio. The contents of five fatty acids in the oils extracted by five techniques from OCPS and determined by GC were as follows: α-linolenic acid (41.12%－51.81%), linoleic acid (15.38%－16.43%), oleic acid (18.93%－27.28%), stearic acid (2.56%－4.01%), and palmitic acid (7.38%－10.77%). Conclusion The results show that STE is the most efficient technology with the highest yield (LE: 0.57%; SE: 1.03%; UE: 0.61%; SFE: 0.80%; STE: 1.17%) and shortest time (LE: 720 min; SE: 360 min; UE: 30 min; SFE: 120 min; STE: 1.5 min) among five tested extraction technologies. It is first reported using STE to extract herbal oil enriched with active fatty acids.

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