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Archive > Volume 3 Issue 3 > 2011,3(3):235-238. DOI:10.3969/j.issn.1674-6384.2011.03.012 Prev Next

Original articles

Smashing Tissue Extraction and HPLC Determination of Paclitaxel and 10-Deacetylbaccatin from Taxus x media

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    摘要:


    Abstract:
    Objective To optimize the extraction technology of Taxus x media by using the contents of Paclitaxel and 10-deacetylbaccatin (10-DAB), two representative active diterpene alkaloids of taxane type from T. x media, as evaluation standard. Methods The smashing tissue extraction (STE) of Paclitaxel and 10-DAB from T. x media, was investigated by comparing with ultrasonic extraction (UE) which was one of the modern technologies of extraction. Results STE was more efficient than UE, and the contents of 10-DAB and Paclitaxel in the extracts obtained by STE were higher than those by UE. Conclusion STE is a fast, high-performance, and energy-saving technology for the extraction of diterpene alkaloids of taxane type. STE also provides a simple, component-safe, workable, and highly efficient method for the extraction of active natural product.

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    Project Supported:
    E&T Modern Center for Natural Products of Liaoning Province of China (2008402021)

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