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Archive > Volume 5 Issue 3 > 2013,5(3):217-223. DOI:10.3969/j.issn.1674-6348.2013.03.007 Prev Next

Original articles

Optimization for Preparation of Lemon-catalyzed Ginsenoside Rg3 by Response Surface Method

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    摘要:


    Abstract:
    Objective Ginsenoside Rg3 was prepared by converting protopanaxadiol (PPD)-type saponins using lemon juice as the catalyst. Methods Preparation of ginsenoside Rg3 was optimized by response surface method (RSM) based on a four-factor and five-level central composite design. Results The optimal yield of ginsenoside Rg3 was predicted to be 75.98% in the combination of the factors (PPD-type saponins concentration of 23.6 mg/mL, lemon juice concentration of 97.6% at 85.7 ℃ for 130.0 min) through the canonical analysis and ridge analysis with maximum responses. Under the optimum conditions, the actual yield of ginsenoside Rg3 was 75.57%. Conclusion RSM is effective to optimize the preparation of ginsenoside Rg3 by lemon-catalyzed PPD-type saponins. The achievement for the preparation of ginsenoside Rg3 would promote the development of health-care production.

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    Project Supported:
    Bureau of Science and Technology of Jilin City (201222316)

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