Determination of Protopine in Rat Brain Tissues by RRLC-ESI/Q-TOF-MS Method

Pei-xiang Wang; Yong-hui Li; Yan-jing Li; Ting Geng; Ming-li Li; Gang Ding; Zhen-zhong Wang; Wei Xiao; Pei-xiang Wang; Yong-hui Li; Yan-jing Li; Ting Geng; Ming-li Li; Gang Ding; Zhen-zhong Wang; Wei Xiao

Archive > Volume 6 Issue 2 > 2014,6(2):125-130. DOI:10.1016/S1674-6384(14)60019-8 Prev Next

Original articles

Determination of Protopine in Rat Brain Tissues by RRLC-ESI/Q-TOF-MS Method

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Abstract:

Objective To analyse the quantification of protopine from Corydalis Decumbentis Rhizoma (CDR) extract in brain tissues of rats. Methods A rapid, sensitive, and accurate analytical method based on rapid resolution liquid chromatography (RRLC) coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (Q-TOF-MS) was developed for the quantification of protopine in brain of rats. A simple liquid-liquid extraction process was employed for the sample preparation. Chromatographic separation was achieved using 1.8 μm porous particle columns. Results The calibration curve of protopine was linear in the range of 12–897 ng/mL. The relative standard deviations of intra- and inter-day precision values were less than 10%. The extraction recoveries were 96.4%, 99.6%, and 98.5%, for protopine at the concentration of 598.0, 119.6, and 12.0 ng/mL, respectively, and internal standard (1.27 μg/mL) was (98.60 ± 0.02)%. Conclusion The validated method is successfully applied for the determination of protopine in brain tissue of rats after ig administration of CDR extract.

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National Science and Technology Major Project “Creation of Major New Drugs” from China (2011ZX09401-097)

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