Simultaneous Determination of Four Substances in Plasma and Dermal Microdialysates of Guinea Pig after Different Acupoints Administration of Fufang Baijiezi Gel

Guo-hua Wang; Guo-hua Wang

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Simultaneous Determination of Four Substances in Plasma and Dermal Microdialysates of Guinea Pig after Different Acupoints Administration of Fufang Baijiezi Gel

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Abstract:

Objective To study the pharmacokinetic properties of Fufang Baijiezi Gel (FBG) after differernt acupoints administration. Methods A sensitive liquid chromatography tandem mass spectrometry method (LC-MS) was developed and validated to determine sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin in plasma of guinea pig and dermal microdialysates simultaneously. Microdialysates were separated on a Ultimate? XB-Phenyl analytical column (150 mm × 2.1 mm, 5μm) and detected by electrospray ionization (ESI) in selected ion monitoring (SIM) mode. The method was validated in terms of selectivity, linearity, sensitivity, and recovery. A signi?cant difference was observed in main pharmacokinetic parameters of Cmax, tmax, and AUC between acupoints administration and nonacupoints administration. Acupoints administration resulted in a more obvious increase in bioavailability of sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin than nonacupoints administration according to the above LC-MS assay method.

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National Natural Science Foundation of China (No. 81001649)

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