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Archive > Volume 10 Issue 1 > 2018,10(1):86-94. DOI:10.1016/j.chmed.2018.01.006 Prev Next

中國(guó)不同地區(qū)三棱的化學(xué)特征比較

Original articles

Comparative chemical characters of Sparganii Rhizoma from different regions in China

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    摘要:
    目的：通過色譜分析比較不同地區(qū)三棱的化學(xué)特征，并建立LC/MS定量測(cè)定方法用以輔助三棱藥材質(zhì)量評(píng)價(jià)。方法：采用優(yōu)化后的HPLC-PDA色譜條件對(duì)20批三棱進(jìn)行HPLC指紋圖譜分析，并以三棱指紋圖譜所有峰面積區(qū)域?yàn)橐罁?jù)進(jìn)行主成分分析（PCA）和正交偏最小二乘判別分析（OPLA-DA）和聚類分析（HCA）。其中部分共有峰通過UFLC-QTRAP-MS進(jìn)行定量分析。結(jié)果：測(cè)得三棱HPLC指紋圖譜的相似度在0.511-0.973范圍內(nèi)波動(dòng)，說明在化學(xué)成分方面20個(gè)產(chǎn)區(qū)的三棱樣品間存在不同程度的差異。PCA、OPLS-DA和HCA可將樣品分成與其地理分布有關(guān)聯(lián)并具不同化學(xué)特征的五個(gè)亞組。HPLC指紋圖譜中共31個(gè)共有峰，其中8個(gè)被鑒別并定量。結(jié)果顯示產(chǎn)于中國(guó)東部的三棱含有相對(duì)較多的酚酸類和黃酮類成分，大致符合基于HPLC指紋圖譜的樣品分類結(jié)果。結(jié)論：證實(shí)了地理分布與植物內(nèi)在化學(xué)成分組成存在一定聯(lián)系，為傳統(tǒng)中醫(yī)藥理論中的“道地”提供了依據(jù)。所建立的LC/MS定量方法靈敏快速，可用于對(duì)三棱藥材的進(jìn)行質(zhì)量控制。

    Abstract:
    Objective To compare the chemical characters of Sparganii Rhizoma from different areas via chromatographic analysis and to establish a sensitive LC/MS method for quality assessment of Sparganii Rhizoma. Methods Under the optimised HPLC-PDA chromatographic conditions, twenty batches of Sparganii Rhizoma were analyzed by HPLC fingerprints. Principal component analysis (PCA), orthogonal projections to latent structures discriminant analysis (OPLS-DA) and hierarchical cluster analysis (HCA) were performed based on all peak areas of Sparganii Rhizoma fingerprints. Meanwhile, part of common peaks were subsequently quantified by UFLC-QTRAP-MS. Results The similarity values of HPLC fingerprints fluctuated in a wide range of 0.511-0.973, which showed variable differences of chemical characters among Sparganii Rhizoma from twenty habitats. PCA, OPLS-DA and HCA indicated that samples could be divided into five groups with different chemical characters, which generally corresponded with their geographical distributions. A total of 31 peaks in HPLC fingerprints were marked, and eight of them were identified and quantified. The quantitative result was generally in agreement with the classifications based on HPLC fingerprints, which indicated that Sparganii Rhizoma samples from eastern China mostly contained more contents including phenolic acids and flavonoids. Conclusion This study not only proved that there are relationships between geographic distributions and internal chemical compositions of plants, which could provide evidence to the traditional Chinese medicine concept “geo-authentic”, but also supplied a sensitive and rapid simultaneous quantitive method for the quality estimation of Sparganii Rhizoma.

    關(guān)鍵詞:
    化學(xué)特征;地理分布;同時(shí)測(cè)定;三棱

    Keywords:


    Project Supported:
    National Natural Science Foundation of China (81073002); the National Science and Technology Special Fund of China (2015FY111500-110).

    Clc Number:

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