兩色金雞菊有效成分抑制體內(nèi)外的代謝紊亂

Bao-ping Jiang1; Qiu-yue Lv1; Jia-mei Xiang1; Liang Le 2; 3; Ke-ping Hu1; Li-jia Xu1; Pei-gen Xiao1; Bao-ping Jiang1; Qiu-yue Lv1; Jia-mei Xiang1; Liang Le 2; 3; Ke-ping Hu1; Li-jia Xu1; Pei-gen Xiao1

Archive > Volume 10 Issue 2 > 2018,10(2):156-167. DOI:10.1016/j.chmed.2018.03.004 Prev Next

兩色金雞菊有效成分抑制體內(nèi)外的代謝紊亂

Original articles

Inhibition of metabolic disorders in vivo and in vitro by main constituent of Coreopsis tinctoria

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摘要:

目的:為了探討兩色金雞菊乙酸乙酯提取物（EAEC）對高糖高脂引起誘導的大鼠胰島素抵抗的影響。方法：采用高糖高脂飼養(yǎng)SD雄性大鼠并灌胃給與EAEC 8周。給藥結(jié)束后，采取血液測定血糖、胰島素、血脂等指標，收集肝臟組織進行HE染色觀察病理變化。細胞實驗中，利用高糖處理HepG2細胞制備胰島素抵抗模型，使用兩色金雞菊有效成分馬里苷處理細胞，觀察細胞糖攝取、代謝組學等。結(jié)果：高糖高脂明顯導致大鼠血糖、胰島素、血清TC，TG，LDL-C水平顯著升高，而給與EAEC則明顯改善血糖代謝和血脂代謝。高糖處理的HepG2細胞顯著降低肝糖原的合成，增加PEPCK,G6Pase和三羧酸循環(huán)有關(guān)的酶的蛋白水平，導致HepG2的細胞代謝紊亂，而馬里苷則明顯改善高糖誘導的細胞代謝紊亂。結(jié)論：EAEC明顯改善大鼠胰島素抵抗，而馬里苷能改善HepG2細胞代謝紊亂。

Abstract:

Objective To investigate the effects of the ethyl acetate extract of Coreopsis tinctoria (EAEC) on insulin resistance (IR) in rats fed a high-fat diet. Methods Male Sprague-Dawley (SD) rats were fed a HFD (60% fat) supplemented with EAEC for 8 weeks. The administration of EAEC to the rats with HFD-induced insulin resistance reduced hyperglycemia, plasma levels of insulin, and steatosis in the liver. Metabolomic study was used to analyse the metabolic levels of the high glucose-treated cells, control cells and marein-treated cells. Results High glucose and high fat conditions caused a significant increase in blood glucose, insulin, serum TC, TG and LDL-C levels, leading to abnormal IR in rats. However, treatment with EAEC protects against HFD-induced IR by improving the fasting serum glucose homeostasis and lipid homeostasis. The high glucose conditions significantly decreased glycogen synthesis and increased PEPCK, G6Pase and Krebs cycle-related enzyme protein levels, leading to an abnormal metabolic state in HepG2 cells. However, treatment with marein improved IR by increasing glucose uptake and glycogen synthesis and by downregulating PEPCK and G6Pase protein levels. The statistical analysis of the HPLC/MS data demonstrated that marein could restore the normal metabolic state. Conclusion The results revealed that EAEC ameliorates IR in rats, and marein has the potential to improve IR by ameliorating glucose metabolism disorders.

關(guān)鍵詞:

代謝組學、馬里苷、胰島素抵抗、代謝產(chǎn)物、三羧酸循環(huán)

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This work was supported by funding from the National Natural Science Foundation of China (Grant 81573576, 81703223) and the China Postdoctoral Science Foundation (2017M611127).

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