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Archive > Volume 10 Issue 2 > 2018,10(2):176-182. DOI:10.1016/j.chmed.2018.03.008 Prev Next

基于DNA條形碼和高效液相色譜法鑒定及質量評價三七和越南三七

Original articles

Identification and quality analysis of Panax notoginseng and Panax vietnamensis var. fuscidicus through integrated DNA barcoding and HPLC

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    摘要:
    目的：三七（Panaxnotoginseng（Burk.）F. H. Chen）以其塊根及根莖的治療效果而聞名，越南三七(P. vietnamensis var.fuscidicus)被當地居民稱為“野三七”或“越南三七”，已在市場上流通。由于其形態(tài)相似，可能會引起臨床用藥的安全問題，因而迫切需要一種快速而有效的方法進行鑒別。 方法：本研究采用DNA條形碼技術對三七與越南三七進行鑒定，利用高效液相法評價二者不同組織三七皂苷R1和人參皂苷（即Rb1、Rg1、Re、Rd、Rc和Rb2）的差異。 結果：條形碼結果表明三七與越南三七被聚成不同分支，越南三七與越南人參（Panaxvietnamensis）聚為一支，相似度為98%。三七與越南三七的化學成分組成在根中相似，而在須根、莖、葉和花中種類及含量均有差異。 結論：利用ITS2能快速、有效鑒別三七和越南三七；HPLC結果表明三七和越南三七的藥理活性可能存在差異。

    Abstract:
    Objective Root or rhizome of Panax notoginseng (Sanqi) is known for its eutherapeutic effects. Panax vietnamensis var. fuscidicus, called Yesanqi or Yuenan sanqi by local residents, is also commercially available. They are similar in morphology, leading to serious safety problems in clinical medication. It is necessary to find the rapid and efficient methods to identify them. Methods P. notoginseng and P. vietnamensis var. fuscidicus were identified by DNA barcoding based on the ITS2 sequence. Notoginsenoside R1 and ginsenosides (Rb1, Rg1, Re, Rd, Rc, and Rb2) were analyzed in the roots, fibrils, stems, leaves, and flowers of P. notoginseng and P. vietnamensis var. fuscidicus using high-performance liquid chromatography (HPLC). Results P. notoginseng and P. vietnamensis var. fuscidicus were separated into branches of divergent clusters, and P. vietnamensis var. fuscidicus and Panax vietnamensis were clustered into a clade with 98% similarity according to DNA barcoding analysis. The chemical compositions of P. notoginseng and P. vietnamensis var. fuscidicus were similar in roots; while their compositions and contents of the notoginsenoside R1 and ginsenosides in flowers, leaves, stems, and fibrils were different. Conclusion ITS2 is a rapid and efficient method to identify P. notoginseng and P. vietnamensis var. fuscidicus. HPLC analysis indicated that pharmacological action might be different between P. notoginseng and P. vietnamensis var. fuscidicus.

    關鍵詞:
    DNA條形碼;高效液相色譜法;鑒定;三七

    Keywords:


    Project Supported:
    We thank Prof. Zhongjian Chen for the identification of voucher speciments. This study was supported by the grants from the National Natural Science Foundation of China (No. 81603238), Beijing Nova Project (No. xx2018014), and the Major Science and Technology Programs in Yunnan Province (No. 2016ZF001-001).

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