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Archive > Volume 10 Issue 2 > 2018,10(2):183-189. DOI:10.1016/j.chmed.2018.01.009 Prev Next

槲皮苷對(duì)大鼠骨髓間充質(zhì)干細(xì)胞（rBMSCs）成骨分化和成脂分化的分子調(diào)控機(jī)制研究

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Molecular Mechanism of Quercitrin on Osteogenic Differentiation and Adipogenic Differentiation of Rat Bone Marrow Stromal Stem Cells（rBMSCs）

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    摘要:
    目的：研究槲皮苷對(duì)大鼠骨髓間充質(zhì)干細(xì)胞 ( rBMSCs) 成骨和成脂 方向分化的分子調(diào)控機(jī)制。方法：原代培養(yǎng)SD大鼠rBMSCs，給予0.1 -10µg/mL的槲皮苷對(duì)rBMSCs 的成骨性分化進(jìn)行干預(yù)后，對(duì)堿性磷酸酶( ALP) 活性進(jìn)行測(cè)定，茜素紅進(jìn)行染色，并且采用RT-PCR技術(shù)檢測(cè)成骨細(xì)胞分化標(biāo)志物（Runx2, BMP-2, OSX）mRNA的表達(dá)；給予0.1 -10µg/mL的槲皮苷對(duì)rBMSCs 的成脂性分化進(jìn)行干預(yù)后，油紅O進(jìn)行染色，并且采用RT-PCR技術(shù)檢測(cè)成脂細(xì)胞分化標(biāo)志物（PPARγ, C/EBPα, aP2）mRNA的表達(dá)。結(jié)果：槲皮苷顯著提高了成骨誘導(dǎo)后細(xì)胞ALP 的活性，增加了鈣化結(jié)節(jié)數(shù)，并且能上調(diào)成骨分化標(biāo)志物（Runx2, BMP-2, OSX）的mRNA表達(dá)；另一方面槲皮苷顯著減少了成脂誘導(dǎo)后脂滴的形成，并能抑制成脂分化標(biāo)志物（PPARγ, C/EBPα, aP2）mRNA的表達(dá)。結(jié)論: 本研究表明，槲皮苷對(duì) rBMSCs 分化的調(diào)節(jié)具有雙向性，即它在促進(jìn) rBMSCs 成骨性分化的同 時(shí)又抑制rBMSCs 成脂性分化。其分子作用機(jī)制可能與上調(diào)Runx2，BMP-2，OSX mRNA表達(dá)，抑制PPARγ, C/EBPα和 aP2 mRNA表達(dá)有關(guān)。

    Abstract:
    Objective The study was designed to investigate the molecular mechanism of quercitrin on osteogenic differentiation and adipogenic differentiation of rBMSCs. Methods rBMSCs were harvested from SD rats, and determination of alkaline phosphatase (ALP) activity, quantification of mineralization by Alizarin Red S staining, and the mRNA expression of osteogenic differentiation markers (Runx2, BMP-2, and OSX) by RT-PCR after rBMSCs stimulated by osteogenic induction with (0.1-10) μg/mL of quercitrin, quantification of Lipid droplet by Oil Red O staining and the mRNA expression of adipogenic differentiation marker (PPARγ, C/EBPα, and aP2) by RT-PCR after rBMSCs stimulated by adipogenic induction with (0.1-10) μg/mL of quercitrin. Results Quercitrin can up-regulate the mRNA expression of osteogenic differentiation markers (Runx2, BMP-2, and OSX) and increase ALP activity and mineralization after osteogenic induction, on the other hand quercitrin can suppress the mRNA expression of adipogenic differentiation markers (PPARγ, C/EBPα, and aP2) and decrease lipid droplet after adipogenic induction. Conclusion This study suggested that quercitrin not only stimulated osteogenic differentiation but also inhibited adipogenic differentiation of rBMSCs, which was associated with the up-regulation of Runx2, BMP-2, and OSX mRNA expression and the down-regulation of PPARγ, C/EBPα, and aP2 mRNA expression.

    關(guān)鍵詞:
    槲皮苷;骨髓間充質(zhì)干細(xì)胞;成骨分化，成脂分化

    Keywords:


    Project Supported:
    This work was supported by National Natural Science Foundation of China (No. 81160508); Science and Technology Project of Jiangxi Province (KJLD14058).

    Clc Number:

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