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Archive > Volume 10 Issue 2 > 2018,10(2):222-229 Prev Next

血塞通與阿司匹林聯(lián)合用藥對(duì)大鼠腦缺血再灌注損傷的保護(hù)作用

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Protective effects of combination of Xuesaitong and aspirin on cerebral ischemia and reperfusion injury in rats

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    摘要:
    目的：探討血塞通與阿司匹林聯(lián)合用藥對(duì)大鼠腦缺血再灌注損傷的保護(hù)作用及其作用機(jī)制。 方法：將150只雄性SD大鼠隨機(jī)分為假手術(shù)組、大腦中動(dòng)脈缺血再灌注（MCAO/R）模型組、血塞通組、阿司匹林組、血塞通+阿司匹林組。預(yù)給藥7天，末次給藥1h后制作MCAO/R模型。按照Longa的5級(jí)評(píng)分方法進(jìn)行神經(jīng)功能缺損評(píng)分；通過(guò)測(cè)量腦含水量觀察腦水腫；TTC染色測(cè)定腦梗死體積。采用試劑盒測(cè)定血清、大腦皮層和海馬組織中MDA的含量，SOD、CAT和GSH-Px的活性；ELISA檢測(cè)IL-1β、IL-4、IL-6、IL-10、TNF-α、MCP-1的水平；Western blot 測(cè)定分析大腦皮層組織中Nrf2、HO-1、IκBα、NF-κB/p65蛋白的表達(dá)情況。 結(jié)果：血塞通組、阿司匹林組、血塞通與阿司匹林合用組均能顯著改善MCAO/R大鼠神經(jīng)功能缺損，減輕腦水腫，減少腦梗死體積，降低血清、大腦皮層和海馬組織中的犬尿素、MDA、MCP-1、TNF-α、IL-1β和IL-6水平，降低MDA含量，增加SOD、CAT、GSH-Px、IL-4和IL-10水平。此外，血塞通與阿司匹林單用及合用均能降低NF-κB/p65在細(xì)胞核內(nèi)的表達(dá)，同時(shí)增加IκBα，HO-1和細(xì)胞核內(nèi)Nrf2的表達(dá)。且血塞通與阿司匹林聯(lián)合用藥對(duì)CIRI大鼠的保護(hù)作用效果優(yōu)于二者單用。 結(jié)論：血塞通與阿司匹林聯(lián)合用藥能明顯抑制MCAO/R大鼠血清，大腦皮層和海馬的氧化應(yīng)激和炎癥反應(yīng)，且血塞通與阿司匹林合用的作用效果優(yōu)于二者單用。血塞通與阿司匹林聯(lián)合用藥對(duì)大鼠腦缺血再灌注損傷具有協(xié)同治療作用，值得進(jìn)一步臨床研究。

    Abstract:
    Objective To investigate the protective effects of the combination of Xuesaitong (XST) and aspirin on cerebral ischemia and reperfusion injury (CIRI) in rats, and further explore the underlying mechanisms. Methods A total of 150 male Sprague-Dawley (SD) rats were randomly divided into five groups with 30 rats in each group: sham group, middle cerebral artery occlusion/reperfusion (MCAO/R) model group, XST group, aspirin group, and XST + aspirin group. Rats were pretreated with XST, aspirin, or XST + aspirin for 7 d. One hour after the last administration, a model of CIRI was induced by MCAO/R. Neurological deficits were assessed using Longa’s five-point scale. Cerebral edema was detected by the measurement of brain water content. The volume of cerebral infarction was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), as well as levels of malonaldehyde (MDA) were detected by commercial kits. Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of interleukin-1 (IL-1β), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α), monocyte chemotactic protein 1 (MCP-1), and kynurenine in serum, cerebral cortex, and hippocampus of MCAO/R rats. The protein expression of nuclear factor erythroid 2-related factor (Nrf2), heme oxygenase-1 (HO-1), I-kappa B alpha (IκBα), and nuclear factor kappa B (NF-κB)/p65 in the cortex were analyzed by western blotting. Results Treatment of XST, aspirin, and XST + aspirin significantly alleviated the neurological deficits, cerebral edema, and cerebral infarct volume induced by MCAO/R. Treatment of XST, aspirin, and XST + aspirin also reduced MDA, IL-1β, IL-6,TNF-α, MCP-1, and kynurenine levels, and increased SOD, CAT, GSH-Px, IL-4, and IL-10 levels in serum, cerebral cortex, and hippocampus of MCAO/R rats. Furthermore, treatment of XST, aspirin, and XST + aspirin decreased the expression of nuclear NF-κB/p65 and increased the expression of IκBα, nuclear Nrf2, and HO-1. Importantly, the combination of XST and aspirin enhanced the protective effects of XST or aspirin treatment alone on CIRI in rats. Conclusion The combination of XST and aspirin significantly inhibited oxidative stress and inflammation in serum, cerebral cortex, and hippocampus of MCAO/R rats. The combination of XST and aspirin exerted more protective effects than XST or aspirin treatment alone. The combination of XST and aspirin might provide the synergistic therapeutic effects on CIRI, and deserve further clinical investigation.

    關(guān)鍵詞:
    血塞通;阿司匹林;腦缺血再灌注損傷;氧化應(yīng)激;炎癥反應(yīng)

    Keywords:


    Project Supported:
    This work is supported by the Major collaborative innovation projects of Chinese Academy of Medical Sciences (No. CAMS-2016-I2M-1-010，2016-I2M-1-012).

    Clc Number:

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