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Archive > Volume 10 Issue 3 > 2018,10(3):263-268 Prev Next

丹參毛狀根內(nèi)SmWRKY42-like的轉(zhuǎn)錄激活與亞細(xì)胞定位以及對(duì)外源赤霉素與乙烯處理的響應(yīng)

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Transcriptional activity and subcellular location of SmWRKY42-like and its response to gibberellin and ethylene treatments in Salvia miltiorrhiza hairy roots

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    摘要:
    目的: 外源赤霉素（GA）與乙烯處理能提高丹參中的藥用成分的含量. 有趣的是, 大量報(bào)道表明WRKY 轉(zhuǎn)錄因子在相應(yīng)這些信號(hào)中起著重要的調(diào)控作用。然而， 是否丹參中的SmWRKY 介導(dǎo)了這些信號(hào)仍然很不清楚。方法： qRT-PCR 用于SmWRKY42-like 對(duì)外源GA 與乙烯利(Eth) 處理響應(yīng)的檢測(cè)。SmWRKY42-like 的亞細(xì)胞定位采用基因槍法將目的質(zhì)粒在洋蔥表皮細(xì)胞瞬時(shí)表達(dá)。SmWRKY42-like的自激活實(shí)驗(yàn)在AH109酵母株系內(nèi)驗(yàn)證. 結(jié)果: SmWRKY42-like是丹參WRKY家族內(nèi)的一個(gè)基因。SmWRKY42-like的亞細(xì)胞定位與自激活實(shí)驗(yàn)結(jié)果顯示其蛋白表達(dá)主要富集在細(xì)胞核內(nèi)并且是丹參內(nèi)潛在的轉(zhuǎn)錄因子。與此同時(shí), SmWRKY42-like能顯著地響應(yīng)外源GA與 Eth 處理. 結(jié)論: 以上結(jié)果表明 SmWRKY42-like 作為一個(gè)重要的激素響應(yīng)基因可能在ET與GA 信號(hào)轉(zhuǎn)導(dǎo)途徑中具有潛在的角色。

    Abstract:
    Objective: The exogenous gibberellin (GA) and ethylene (ET) treatment can improve the medicinal ingredients of Salvia miltiorrhiza. Interestingly, many reports pointed out that WRKY transcription factors played an important regulatory role in these treatment responses. However, whether the SmWRKY mediate these treatment signalings in S. miltiorrhiza remains largely elusive. Methods qRT-PCR was used for SmWRKY42-like in response to exogenous GA and ethephon (Eth) treatment. The subcellular location of SmWRKY42-like was transiently transformed into onion epidermal cells by particle bombardment. The self-activating activity of SmWRKY42-like was verified in AH109 yeast strain. Results: SmWRKY42-like was a WRKY family gene in S. miltiorrhiza. The subcellular localization and transcriptional activity results of the SmWRKY42-like protein indicated that SmWRKY42-like mainly enriched in nucleus and might be a transcription factor in S. miltiorrhiza. In the meantime, the SmWRKY42-like gene significantly responded to exogenous GA and Eth treatment. Conclusion: These results collectively indicated the SmWRKY42-like gene functions, as an important hormone-responsive gene, might play a potentially role in ET and GA signaling pathways.

    關(guān)鍵詞:
    丹參, SmWRKY42-like, ET, GA, 亞細(xì)胞定位

    Keywords:


    Project Supported:
    the National Natural Science Foundation of China (81373908 and 81373536)

    Clc Number:

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