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Archive > Volume 10 Issue 3 > 2018,10(3):298-303 Prev Next

正天丸對大鼠海馬內(nèi)嗅區(qū)前穿質(zhì)-齒狀回通路及謝弗側(cè)枝-CA1通路的突觸傳遞作用研究

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Zhengtian Pills accelerated long term potentiation both in schaffer collateral -CA1 and perforant path-dentate gyrus synapses

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    摘要:
    目的：為了研究正天丸對大鼠海馬內(nèi)嗅區(qū)前穿質(zhì)-齒狀回通路及謝弗側(cè)枝-CA1通路的突觸傳遞作用。方法：將SD大鼠隨機(jī)分為五組：空白組，陽性對照組，偏頭痛模型組，正天丸低劑量組及正天丸高劑量組。以皮下注射三硝酸甘油酯 (10 mg/kg) 的大鼠為偏頭痛模型，氟桂嗪 (0.9 mg/kg) 為陽性對照藥，我們采取電生理實驗技術(shù)，研究正天丸對麻醉大鼠海馬內(nèi)嗅區(qū)前穿質(zhì)-齒狀回通路以及正天丸對大鼠離體海馬腦片謝弗側(cè)枝-CA1通路的突觸傳遞作用的影響。結(jié)果：與對照組相比，正天丸 (1.08 g/kg) 對麻醉大鼠海馬內(nèi)嗅區(qū)前穿質(zhì)-齒狀回通路內(nèi)高頻刺激誘導(dǎo)的LTP指標(biāo)，PS幅度有顯著的增強(qiáng)作用；而三硝酸甘油酯 (10 mg/kg) 可以顯著的降低PS的幅度；正天丸 (0.54 g/kg) 和氟桂嗪 (0.9 mg/kg)均對PS幅度沒有增強(qiáng)作用。與模型組相比，正天丸 (1.08 g/kg)，正天丸 (0.54 g/kg) 和氟桂嗪 (0.9 mg/kg) 均對PS幅度有顯著的增強(qiáng)作用。在離體海馬腦片實驗中，與對照組相比，正天丸 (1.08 g/kg) 對離體海馬腦片謝弗側(cè)枝-CA1通路內(nèi)高頻刺激誘導(dǎo)的LTP指標(biāo)，fEPSP斜率有顯著的增強(qiáng)作用；而三硝酸甘油酯 (10 mg/kg) 可以顯著的降低fEPSP斜率；正天丸 (0.54 g/kg) 和氟桂嗪 (0.9 mg/kg) 均對fEPSP斜率沒有顯著的增強(qiáng)作用。與模型組相比，正天丸 (1.08 g/kg)、正天丸 (0.54 g/kg) 和氟桂嗪 (0.9 mg/kg) 均對fEPSP斜率有顯著的增強(qiáng)作用。結(jié)論：結(jié)合我們之前的研究，可以得出正天丸對TRPV1蛋白和海馬LTP的作用可能是正天丸作為治療偏頭痛藥物的重要分子機(jī)制。

    Abstract:
    Objective To investigate the effects of Zhengtian Pills (ZTP) on long term potentiation (LTP) both in schaffer-CA1 in vitro and perforant path-dentate gyrus (PP-DG) synapses in vivo. Methods Sprague-Dawley rats were randomly divided into five groups: control, positive control, migraine model, low-, and high-dose ZTP groups. Glyceryl trinitrate (10 mg/kg) was injected subcutaneously to make migraine rat model. Flunarizine (0.9 mg/kg) was set as positive control. Extracellular recording technique in vivo was used to record the effects of ZTP on LTP of PP-DG pathway in anesthetized rats; Using extracellular recording technique in vitro, the effects of ZTP on LTP of Schaffer Collateral-CA1 pathway in rat hippocampal slices were investigated. Results Compared to the control group, ZTP (1.08 g/kg) had significantly enhanced population spike amplitude in PP-DG pathway; Glyceryl trinitrate (10 mg/kg) significantly reduced population spike amplitude in PP-DG pathway; Neither ZTP (0.54 g/kg) nor Flunarizine (0.9 mg/kg) had significant effects on LTP in PP-DG pathway. Compared to the model group, ZTP (1.08 g/kg), ZTP (0.54 g/kg), and flunarizine (0.9 mg/kg) had significantly enhanced population spike amplitude in PP-DG pathway. Compared to the control group, ZTP (1.08 g/kg) significantly enhanced field excitatory postsynaptic potential (fEPSP) slope in Schaffer Collateral-CA1 pathway; Glyceryl trinitrate (10 mg/kg) significantly reduced fEPSP slope in Schaffer Collateral-CA1 pathway; Neither ZTP (0.54 g/kg) nor flunarizine (0.9 mg/kg) had significant effects on LTP in Schaffer Collateral-CA1 pathway, Compared to the model group, ZTP (1.08 g/kg), ZTP (0.54 g/kg), and Flunarizine (0.9 mg/kg) had significantly enhanced fEPSP slope in Schaffer Collateral-CA1 pathway. Conclusion: Combined with the previous study, the results gave a clue that the effects of ZTP on TRPV1 and hippocampal LTP or their interactions could be the important molecular mechanisms of ZTP acting as migraine and headache medication.

    關(guān)鍵詞:
    海馬;長時程增強(qiáng)（LTP）;偏頭痛;正天丸（ZTP）

    Keywords:


    Project Supported:
    Ministry of Science and Technology (No. 2013ZX09201022), Scientific Research Common Program of Beijing Municipal Commission of Education (No. KM201 100250), and National Natural Science Foundation of China (grant No. 81373418) for financial support of this project

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