基于化學(xué)計量學(xué)方法的茺蔚子和炒茺蔚子質(zhì)量評價研究

Dong-hui Yan; Xin-dan Liu; Xian-mei Deng; Bin Zhao; Xing-yang Xue; Shu-mei Wang; Ying Zhang; Jiang Meng; Dong-hui Yan; Xin-dan Liu; Xian-mei Deng; Bin Zhao; Xing-yang Xue; Shu-mei Wang; Ying Zhang; Jiang Meng

Archive > Volume 10 Issue 4 > 2018,10(4):388-395. DOI:10.1016/j.chmed.2018.07.006 Prev Next

基于化學(xué)計量學(xué)方法的茺蔚子和炒茺蔚子質(zhì)量評價研究

Original articles

Quality assessment of crude and processed Leonuri Fructus by chemical and color analysis combined with chemometric method

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摘要:

目的：茺蔚子是唇形科植物益母草干燥成熟果實，炒茺蔚子為茺蔚子的炮制加工品，二者在中國和其他亞洲國家有數(shù)千年的臨床應(yīng)用歷史。本研究的目的是揭示茺蔚子和炒茺蔚子之間的區(qū)別。方法：聯(lián)合采用人工智能色彩色差計、高效液相-蒸發(fā)光散射（HPLC-ELSD）和氣相色譜-氫火焰檢測（GC-FID）等方法檢測分析茺蔚子和炒茺蔚子多成分指標(biāo)及色彩色差值的不同。應(yīng)用人工智能色彩色差計CR-410測定樣品的顏色參數(shù)，應(yīng)用HPLC-ELSD和GC-FID測定水蘇堿和六種脂肪酸的含量，并通過方差分析（ANOVA）、主成分分析（PCA）、層次聚類分析（HCA）和Kendall相關(guān)性測試等進(jìn)行數(shù)據(jù)分析。結(jié)果：以水蘇堿和六種脂肪酸成分含量和顏色為指標(biāo)，采用PCA和HCA分析顯示茺蔚子和炒茺蔚子分為兩類，說明茺蔚子和炒茺蔚子之間顏色和多成分含量具有顯著性區(qū)別。結(jié)論：基于HPLC、GC和人工智能色差計法結(jié)合化學(xué)計量學(xué)方法可用于CLF和PLF的綜合質(zhì)量評價。

Abstract:

Objective: Crude Leonuri Fructus (CLF), the fruits of the Leonurus japonicus, and processed Leonuri Fructus (PLF) by stir-baking as the important Chinese herbal medicines, have been used in China and other Asian countries for thousands of years. The objective of this research is to reveal the difference between CLF and PLF. Methods: The sensory technologies of the colorimetry, sensitive and validated HPLC-ELSD and GC combined with flame ionization detector (GC-FID) were employed to discriminate CLF and its processed product PLF. The color parameters of the samples were determined by colorimetric instrument CR-410. Moreover, the content of stachydrine and six fatty acids were determined by HPLC and GC. Subsequently, analysis of variance (ANOVA), principal components analysis (PCA), hierarchical cluster analysis (HCA), and Kendall’s correlation test were performed for data analysis. Results: The CLF and PLF were divided into two categories by PCA and HCA in terms of their component content and color. The results distinctly demonstrated significant changes in color and the content of indicative components between CLF and PLF. Conclusion: The study revealed that HPLC, GC, and colorimetric method in combination with chemometric method could be used as comprehensive quality evaluation for CLF and PLF.

關(guān)鍵詞:

顏色分析;成分檢測;茺蔚子;層次聚類分析;主成分分析

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