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Archive > Volume 10 Issue 4 > 2018,10(4):445-450. DOI:10.1016/j.chmed.2018.08.002 Prev Next

桂皮醛對免疫抑制BALB/c小鼠肺白念珠菌細(xì)胞壁(1,3)-β-D葡聚糖的影響

Original articles

Effect of cinnamaldehyde on 1, 3-β-d-glucans in cell wall of Candida albicans in immunosuppressed BALB/c mice

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    摘要:
    目的 侵襲性肺念珠菌感染發(fā)病率高、治療棘手、預(yù)后差、死亡率高，研究桂皮醛對白念珠菌細(xì)胞壁(1, 3)-β-D-glucans的影響，為研發(fā)新型靶向抗真菌藥物提供依據(jù)。方法 對免疫抑制BALB/c小鼠經(jīng)鼻腔灌注1x107 cfu/ml 50ul白念珠菌菌懸液，口服桂皮醛240mg.kg-1. d-1，連續(xù)14d后，取肺組織標(biāo)本進行 (1, 3)-β-D-glucans測定及透射電鏡觀察，以氟康唑為對照。結(jié)果 肺組織 (1, 3)-β-D-glucans（1160.62±89.65 pg/ml），氟康唑?qū)φ战M（4285.87±215.62 pg/ml），兩組之間對比較P＜0.01有統(tǒng)計學(xué)差異。肺組織電鏡顯示, 白念珠菌細(xì)胞壁外2-3層（1,3）-β-D葡聚糖層，胞壁溶解脫落,導(dǎo)致胞壁嚴(yán)重缺損，但細(xì)胞膜清晰、完整。結(jié)論 桂皮醛對肺白念珠菌細(xì)胞壁（1, 3）- β-D-glucans具有顯著影響，但細(xì)胞膜不受影響。說明，桂皮醛有獨特的抗白念珠菌細(xì)胞壁(1, 3)-β-D -glucans效應(yīng)。

    Abstract:
    Objective: Invasive pulmonary candidiasis is a disease with high incidence, difficult treatment, poor prognosis, and high mortality. The present study analyzed the influence of cinnamaldehyde on 1,3-β-D-glucans in the cell wall of Candida albicans in order to provide a theoretical basis for the research of antifungal drugs. Methods: An immunosuppressed BALB/c mouse model with invasive pulmonary candidiasis was established by nasal perfusion of 50 μL of C. albicans suspension (107 cfu/mL). 1,3-β-D-glucans examination and electron microscopy were carried out. Fluconazole was used as the control. Results: Cinnamaldehyde was administered at a dose of 240 mg/kg/d for 14 consecutive days, and the measured value of 1,3-β-D-glucans was (1160.62 ± 89.65) pg/mL, whereas that of fluconazole was (4285.87 ± 215.62) pg/mL. The difference between the two groups was statistically significant (P < 0.05). Electron microscopy observation indicated that the 2?3 layers outside the cell wall of C. albicans (1,3-β-D-glucans layer) were rough, deformed, and incomplete, although the cell membrane was clear and intact. Conclusion: Cinnamaldehyde demonstrated special efficacy on 1,3-β-D-glucans in the cell wall of C. albicans.

    關(guān)鍵詞:
    桂皮醛;白念珠菌;侵襲性肺部感染;真菌細(xì)胞壁;(1,3)-β-D-glucans;靶向藥物

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