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Archive > Volume 11 Issue 1 > 2019,11(1):52-59. DOI:10.1016/j.chmed.2018.09.006 Prev Next

基于二維DNA條形碼標(biāo)識(shí)系統(tǒng)鑒定血藤類藥材

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QR code labeling system for Xueteng-related herbs based on DNA barcode

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    摘要:
    目的：血藤類藥材除了包括《藥典》收載的雞血藤、大血藤和滇雞血藤以外，還包括其他皮色發(fā)紅或鮮品被斬?cái)鄷r(shí)流出紅色汁液的藤類藥材。由于藥材性狀及中文名稱相似，導(dǎo)致其在臨床使用和商業(yè)流通中容易引起混淆。因此，亟需建立一種準(zhǔn)確的藥材鑒別標(biāo)識(shí)系統(tǒng)，從而快速、有效的區(qū)分不同的血藤類藥材。方法：對(duì)76份血藤類藥材的ITS2序列進(jìn)行了分析，并基于自行編寫的代碼和開源代碼PHP QR code的編碼方式將它們轉(zhuǎn)換成二維DNA條形碼圖片。此外，基于獲得的ITS2序列建立了血藤類藥材的ITS2條形碼參考數(shù)據(jù)庫，用于25份市售血藤類藥材的檢測(cè)。結(jié)果：不同血藤類藥材的ITS2序列長(zhǎng)度為207-235 bp，GC含量為57.5%-71.0%。NJ系統(tǒng)進(jìn)化樹結(jié)果表明雞血藤、大血藤和滇雞血藤分別各自聚為一支，可有效區(qū)分。BLAST鑒定結(jié)果顯示，其準(zhǔn)確率為100%。生成的二維DNA條形碼圖片直觀，準(zhǔn)確的展示了不同血藤類藥材的ITS2序列特征。25份市售藥材的鑒定結(jié)果顯示，其中22份與標(biāo)簽標(biāo)識(shí)一致，3份標(biāo)識(shí)為“滇雞血藤”的藥材，經(jīng)鑒定為“雞血藤”。結(jié)論：二維DNA條形碼標(biāo)識(shí)系統(tǒng)能夠準(zhǔn)確有效地區(qū)分市場(chǎng)上不同的血藤類藥材，可應(yīng)用于藥材的流通監(jiān)管。

    Abstract:
    Objective Xueteng-related herbs include Jixueteng, Daxueteng, Dianjixueteng, and other medicinal plant stems that release reddish-brown juices when cut. However, similarity in phonetic spelling and sound leads to their misuse in clinic or commercial circulation. Accurate labeling is imperative as well as effective species identification. Methods In this study, the ITS2 sequences of 76 samples of Xueteng-related herbs were obtained and analyzed to identify them. And then they were converted into QR codes using the open source PHP QR code. Also, a DNA barcode reference library was established according to these sequences and was used to authenticate the 25 samples of Xueteng-related herbs collected from the market. Results The lengths of the ITS2 sequences of different Xueteng-related herbs ranged from 207 to 235 bp and the GC contents were 57.5%?71.0%. Jixueteng, Daxueteng, and Dianjixueteng were clustered into three clades respectively in the neighbor-joining (NJ) phylogenetic tree, and the efficiency of the BLAST method was 100%. The ITS2 sequences of different Xueteng-related herbs were presented vividly and specifically in QR code. Twenty-two of all 25 commercial samples were consistent with the original labels, whereas three samples marked “Dianjixueteng” were authenticated as “Jixueteng”. Conclusion QR code labeling system based on DNA barcode is an effective labeling system of Xueteng-related herbs for their circulation regulation.

    關(guān)鍵詞:
    血藤類藥材; ITS2; DNA標(biāo)識(shí)系統(tǒng); 二維碼; 鑒定

    Keywords:


    Project Supported:
    We thank Dr. Yan Lu from Department of Pharmacognosy, Fudan University for providing some experimental samples. This research was supported by the CAMS Innovation Fund for Medical Sciences (CIFMS, 2016-I2M-3-016) and the Key Projects of the National Science and Technology Pillar Program (No. 2011BAI07B08).

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