梓醇和水蘇糖在治療STZ-HFD誘導(dǎo)的糖尿病上協(xié)同作用：協(xié)同調(diào)節(jié)血糖、血脂水平和肝腎功能

Xiao-fang Chen; Deng-qun Liao; Zhen-xian Qin; Xian-en Li; Xiao-fang Chen; Deng-qun Liao; Zhen-xian Qin; Xian-en Li

Archive > Volume 11 Issue 1 > 2019,11(1):70-77. DOI:10.1016/j.chmed.2018.05.006 Prev Next

梓醇和水蘇糖在治療STZ-HFD誘導(dǎo)的糖尿病上協(xié)同作用：協(xié)同調(diào)節(jié)血糖、血脂水平和肝腎功能

Original articles

Synergistic interactions of catalpol and stachyose in STZ-HFD induced diabetic mice: synergism in regulation of blood glucose, lipids, and hepatic and renal function

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摘要:

目的：地黃塊根常被用來治療糖尿病，其主要藥效成分梓醇和水蘇糖在治療糖尿病和其并發(fā)癥上具有相似的療效。本文我們擬研究梓醇和水蘇糖在治療糖尿病上是否具有協(xié)調(diào)效應(yīng)。方法：用鏈脲霉素加喂高糖高脂飼料誘導(dǎo)糖尿病C57BL/6老鼠，設(shè)對照模型組和6個給藥組：二甲雙胍組，梓醇單獨給藥組，水蘇糖單獨給藥組，梓醇和水蘇糖按1:1、 1:2和 2:1三種配伍給藥組。口服給藥6周后取血液、腎和肝組織分別進行血脂水平、腎肝功能指數(shù)和肝臟病理組織學(xué)分析。結(jié)果：結(jié)果表明梓醇和水蘇糖及其1:1、 1:2和 2:1配伍都能有效降低糖尿病老鼠的體重、血糖和肝腎重指數(shù)及調(diào)控血脂TC、 HDL-c和TG的含量、肝臟的ALT 和 TBA含量。與模型組相比，梓醇及其與水蘇糖配伍為2：1都能顯著提高肝臟白蛋白的含量。梓醇和水蘇糖及其3個配伍都能顯著提高尿肌酐濃度(Ucr)，改善腎臟功能；而在血肌酐(Scr)和肌酐清除速率(Ccr)這兩個指標(biāo)上幾種給藥并沒有一致的調(diào)控作用?？傮w來說梓醇和水蘇糖1:1配伍對肝腎重指數(shù)及ALT、Ucr、 Scr和 Ccr具有較好的調(diào)節(jié)效果，梓醇和水蘇糖配合給藥尤其按1：1配伍對改善糖尿病并發(fā)癥表現(xiàn)出與二者單獨給藥接近或者更好的效果。結(jié)論：基于以上結(jié)果，我們認(rèn)為梓醇和水蘇糖在治療STZ/HFD 誘導(dǎo)發(fā)生的糖尿病上具有協(xié)同效應(yīng)。本文的研究結(jié)果為開發(fā)地黃塊根的有效化合物治療糖尿病及研究地黃治療糖尿病的綜合機理方面提供了思路和技術(shù)支持。

Abstract:

Objective Rehmanniae Radix has been traditionally used to treat diabetes. Catalpol (CAT) and stachyose (STA) are two of the main bioactive compounds in Rehmannia Radix and found to have similar therapeutic effects on diabetes and its complications. In this paper, we aimed to investigate whether there were synergistic therapeutic effects of CAT and STA on diabetes. Methods Streptozotocin (STZ) with the feeding of high-sugar-high-fat diet (HFD) was applied to induce diabetic C57BL/6 mice. STZ-HFD induced diabetic mice were then divided into model and six medical-treated groups: metformin (MET), STA, CAT, and three combinations of CAT:STA (1:1, 1:2, 2:1). Blood, liver, and kidney samples were isolated after six-week oral administration for biochemical assays of serum lipids, the indicators of kidney and liver functions and HE staining for liver tissues. Results It turned out that CAT, STA and their three combinations (1:1, 1:2, 2:1) could effectively control body weight, blood glucose, kidney weight and liver weight index and well regulate levels of TC, HDL-c, TG, ALT, and TBA. In addition, CAT and its combination with STA at the ratio of 2:1 could significantly improve albumin content, compared to that in model group. STA and CAT and their combinations showed the improvements on kidney function in terms of urinary creatinine (Ucr). However, there were no such consistent observations on serum creatinine (Scr) and creatinine clearance rate (Ccr). The combination of CAT and STA at the ratio of 1:1 exhibited the better adjusting effects on kidney weight and liver weight indexes and the levels of ALT, Ucr, Scr, and Ccr. Our results demonstrated that the combinations of CAT and STA especially 1:1 showed similar or better improvements on diabetes-associated complications, compared to the sole CAT or STA treatment. Conclusion Thus, we concluded that there were synergistic therapeutic effects between CAT and STA on STZ/HFD-induced type 2 diabetes. This project provided insights and technical supports for the innovation of discovering bioactive constituents in Rehmannia Radix and studying its integrative mechanism in curing diabetes.

關(guān)鍵詞:

梓醇; 高糖高脂飼料; 地黃; 水蘇糖;協(xié)同作用; 鏈脲霉素誘導(dǎo)糖尿病

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This work was financially supported by the National standardization project of traditional Chinese Medicine (ZYBZH-C-JL-24-03)

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