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Archive > Volume 11 Issue 1 > 2019,11(1):86-91. DOI:10.1016/j.chmed.2018.10.005 Prev Next

赤芍通過(guò)抗神經(jīng)膠質(zhì)增生及抗氧化保護(hù)局部短暫性腦缺血小鼠海馬部位神經(jīng)的研究

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Neuroprotective effect of Paeoniae Radix Rubra on hippocampal CA1

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    摘要:
    目的：卒中是全球第二大死亡原因。本研究探究赤芍（Paeoniae Radix Rubra， PRR）對(duì)缺血性腦卒中小鼠的神經(jīng)保護(hù)作用。方法：使用小鼠大腦中動(dòng)脈堵塞法構(gòu)建局部腦缺血模型。將實(shí)驗(yàn)動(dòng)物分為4組，假手術(shù)組（Sham 組）、假手術(shù)給藥組（PRR-sham組）、手術(shù)組（Isch組）、手術(shù)給藥組（PRR-Isch組）。采用TTC染色法測(cè)量各組小鼠腦梗死體積；免疫組織化學(xué)染色觀察各組之間小鼠缺血側(cè)大腦海馬區(qū)神經(jīng)元細(xì)胞的凋亡情況以及星形膠質(zhì)細(xì)胞和小膠質(zhì)細(xì)胞的活化程度；采用蛋白印跡法觀察小鼠缺血側(cè)海馬超氧化物歧化酶1（SOD1）、超氧化物歧化酶2（SOD2）、過(guò)氧化氫酶抗體（Catalase）表達(dá)水平。結(jié)果：赤芍可以明顯減少局部腦缺血小鼠的腦梗死體積，并能抑制海馬CA1區(qū)星形膠質(zhì)細(xì)胞和小膠質(zhì)細(xì)胞的活化程度，同時(shí)可以改善由缺血再灌注損傷引起的SOD1、SOD2和Catalase的表達(dá)水平下降情況。結(jié)論：赤芍可以通過(guò)抗膠質(zhì)增生以及抗氧化作用改善局部短暫性腦缺血小鼠的神經(jīng)損傷情況。

    Abstract:
    Objective: Stroke is the second leading cause of death worldwide. This study aimed to investigate the neuroprotective effect of Paeoniae Radix Rubra (PRR) on ischemic stroke of mice. Methods: The focal ischemic stroke model was produced via middle cerebral artery occlusion. The experimental mice were divided into four groups: vehicle-sham group, PRR-sham group, vehicle-ischemia group, and PRR-treated ischemia group. The cerebral infarction volume was detected with TTC staining. The number of neurons in the hippocampal CA1 of the ischemic side, and the activation of astrocytes and microglia were observed via immunohistochemical staining. Western blotting was used to determine the expression changes of SOD1, SOD2, and Catalase protein levels in the hippocampus. Results: PRR significantly reduced the cerebral infarct volume induced by ischemic injury and inhibited the astrocytes and microglia activation in the hippocampal CA1 region. The decreased levels of SOD1, SOD2, and Catalase that was induced by ischemic reperfusion were simultaneously improved after PRR treatment. Conclusion: PRR improved neuronal injuries that were induced by transient cerebral ischemia via inhibiting gliosis and elevating antioxidants.

    關(guān)鍵詞:
    缺血再灌注損傷;神經(jīng)膠質(zhì)增生;神經(jīng)保護(hù);氧化應(yīng)激;赤芍

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    Project Supported:
    This study was supported by Special Financial Grant from the China Postdoctoral Science Foundation (2015T80592), General Financial Grant from the China Postdoctoral Foundation (2014M561720), Key University Science Research Project of Jiangsu Province (16KJA310006) and Yangzhou University Student Academic Science and Technology Innovation Project (X20170835).

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