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Archive > Volume 11 Issue 2 > 2019,11(2):160-168. DOI:10.1016/j.chmed.2018.08.012 Prev Next

基于定量和HPLC指紋圖譜定性結(jié)合化學(xué)計量學(xué)分析的不同初加工方法黨參藥材的化學(xué)質(zhì)量評價

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Quantitative and HPLC fingerprint analysis combined with chemometrics for quality evaluation of Codonopsis Radix processed with different methods

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    摘要:
    目的：研究不同初加工方法對黨參藥材化學(xué)質(zhì)量的影響，為黨參藥材的產(chǎn)地初加工提供數(shù)據(jù)參考。 方法：定量和HPLC指紋圖譜定性結(jié)合化學(xué)計量學(xué)的分析方法用于評價黨參藥材的化學(xué)質(zhì)量。以甘肅省岷縣黨參藥材規(guī)范化種植基地種植的黨參藥材為材料，通過HPLC指紋圖譜的分析及黨參炔苷、黨參多糖、蔗糖、葡萄糖、果糖的含量測定，對烘干、曬干、陰干、硫熏、揉搓等不同初加工方法處理的黨參藥材樣品進行綜合的化學(xué)質(zhì)量評價。 結(jié)果：不同初加工方法處理的黨參藥材樣品的化學(xué)成分存在較大的差異。在黨參藥材的產(chǎn)地初加工過程中，建議采用曬干或陰干的初加工方法，避免采用硫熏的初加工方法。根據(jù)企業(yè)對黨參藥材規(guī)?；跫庸さ男枨?，可采用烘干的初加工方法，烘干溫度建議采用40℃-50℃。另外，基于黨參多糖、葡萄糖和果糖的含量，建議采用揉搓3次的初加工方法，基于黨參炔苷和蔗糖的含量，應(yīng)避免采用揉搓的初加工方法。 結(jié)論：該研究表明不同初加工方法對黨參藥材的化學(xué)質(zhì)量具有較大的影響。研究結(jié)果針對不同的需求推薦了相應(yīng)的初加工方法，為黨參藥材的產(chǎn)地初加工及后續(xù)的合理利用奠定了一定的基礎(chǔ)。

    Abstract:
    Objective: To observe the effect of different processing methods on the quality of Codonopsis Radix and provide data reference for the reasonable processing in the producing areas. Methods: An analytical strategy that combined quantitative and HPLC ?ngerprint analysis with chemometrics was developed for the quality evaluation of Codonopsis Radix. Lobetyolin, polysaccharide, sucrose, glucose, and fructose were detected simultaneously in Codonopsis Radix samples treated with different processing methods including dryer-drying, sun-drying, shade-drying, sulfur fumigation and kneading, etc. Results: The results showed that different processing methods had an obvious impact on the quality of Codonopsis Radix. Sun-drying or shade-drying was recommended, and sulfur fumigation should be avoided. And dryer-drying at 40 °C ? 50 °C was suggested to meet the requirement for large scale processing. In addition, based on the content of polysaccharide, glucose, and fructose, it was advised to knead for three times. As for lobetyolin and sucrose, kneading should be avoided. Conclusions: Our ?ndings demonstrated that processing method had a big impact on the quality of Codonopsis Radix. Based on the results, the appropriate processing methods directed at different requirements were recommended. The study could lay a foundation for the reasonable processing of Codonopsis Radix in producing areas.

    關(guān)鍵詞:
    黨參;指紋圖譜;化學(xué)計量學(xué);初加工方法;化學(xué)質(zhì)量評價

    Keywords:


    Project Supported:
    Beijing Municipal Science & Technology Commission (No. Z161100000116101), and CAMS Initiative for Innovative Medicine (CAMS-I2M-1-010)

    Clc Number:

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