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Archive > Volume 11 Issue 4 > 2019,11(4):412-416. DOI:10.1016/j.chmed.2019.03.013 Prev Next

利用UPLC/Q-TOF-MS/MS主成分分析和T-test探討生半夏與法半夏有毒成分的差異

Original articles

Synergistic effect of α-mangostin on antibacterial activity of tetracycline, erythromycin, and clindamycin against acne involved bacteria

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    摘要:
    目的：從化學(xué)成分探討生半夏與法半夏的差異。方法：制備生半夏和法半夏樣品并采用UPLC/Q-TOF-MS/MS測定，通過主成分分析(PCA)和T檢驗分析研究兩組樣品之間的差別，并確定相應(yīng)的化學(xué)標(biāo)記物。UPLC/Q-TOF-MS/MS可準(zhǔn)確地測定得到荷質(zhì)比和MS/MS 的碎片數(shù)據(jù)，通過參考文獻或數(shù)據(jù)庫對照相應(yīng)數(shù)據(jù)我們可以鑒別相應(yīng)化合物。結(jié)果：甘草苷、甘草素、溶血磷脂酰膽堿(LPC)是最具特征的化學(xué)標(biāo)記物。由于LPC可導(dǎo)致炎癥反應(yīng)的發(fā)生，炮制前后LPC的減少是減毒的原因之一；甘草苷和甘草素有治療炎癥的作用且能減少肝損傷作用，其在炮制后的法半夏中的增加也是減毒的原因。結(jié)論：該方法不僅可探索傳統(tǒng)中藥炮制后減毒的機制還可分別對炮制前后中藥的質(zhì)量進行控制。

    Abstract:
    Objective To evaluate the synergistic effect of α-mangostin with tetracycline, erythromycin, and clindamycin against bacteria involved in acne production. Methods A broth microdilution method was used to determine the minimum inhibitory concentration (MIC) of α-mangostin and a range of antibiotics. Synergistic effects on antibacterial activity were determined based on their own MIC, and then by using a checkerboard method and a time-kill assay at 37 °C for 24 h. Results α-Mangostin exhibited antibacterial activity against Propionibacterium acnes, Staphylococcus aureus, S. epidermidis and S. pyogenes with MIC values of 0.78, 3.13, 0.78, and 6.25 μg/mL, respectively. The results of the checkerboard assay showed that α-mangostin produced synergistic effects with tetracycline, erythromycin and clindamycin against all tested bacteria, with a fractional inhibitory concentration index (FICI) between 0.09 and 0.32. Moreover, time-kill curve data indicated that α-mangostin increased the antibacterial activity of tetracycline, erythromycin and clindamycin. Conclusion These findings suggested that α-mangostin may be used to enhance the antibacterial activity of some antibiotics against bacteria involved in acne production.

    關(guān)鍵詞:
    差異;主成分分析;生半夏與法半夏;毒性成分;T-test; UPLC/Q-TOF-MS/MS

    Keywords:


    Project Supported:
    the National Research Council of Thailand (NRCT)

    Clc Number:

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