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Archive > Volume 14 Issue 4 > 2022,14(4):576-582. DOI:https://doi.org/10.1016/j.chmed.2021.08.006 Prev Next

Original articles

Lithocarpus polystachyus (Sweet Tea) water extract promotes human hepatocytes HL7702 proliferation through activation of HGF/AKT/ERK signaling pathway

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    摘要:


    Abstract:
    Objective: Sweet Tea (ST), derived from the leaves of Lithocarpus polystachyus, is a Chinese folk medicine with wide pharmacological activities. However, the promotive effects of ST water extract on hepatocytes proliferation and its underlying mechanism remains still unknown. In the present study, the beneficial effects of ST water extract on human hepatocytes and its possible mechanism were investigated. Methods: MTT assay was used to detect the safety range of ST; HL7702 cells were divided into four groups: control group, ST low- (50 μg/mL), medium- (200 μg/mL) and high-concentration (800 μg/mL) groups; BrdU ELISA and EDU staining were used to observe DNA content and cell proliferation; Moreover, flow cytometry was applied to analyze the distribution of cell cycle. Furthermore, the expression of cyclin D1, CDK4, HGF/c-Met, Akt, Erk1/2 were detected by Western blot. Results: It was found that ST water extract concentration-dependent promoted human hepatocytes HL7702 cell proliferation within 72 h through accumulating the cells in S phase and G2/M phase. Furthermore, ST water extract up-regulated expression of Cyclin D1 and CDK4 proteins. Moreover, ST water extract not only increased HGF expression and phosphorylation of c-Met level, but also activated the phosphorylation levels of AKT, ERK1/2. Interestingly, both of AKT inhibitor A6730 and ERK1/2 inhibitor U0126 reversed the promotive effects of ST water extract, which further confirmed that activation of AKT and ERK1/2 were involved. Conclusion: The findings reveal that ST water extract promoted HL7702 cells proliferation through the stimulation of cell cycle mediated by activating the AKT- and ERK1/2-related pathway.

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    Project Supported:
    This work was supported by Natural Science Foundation of China (No. 81760727), Science and Technology Support Plan of Guizhou Province (No. Supporting Science and Technology Cooperation of Guizhou Province [2020]1Y010), Fund Guidance Project of Zunyi Science and Technology Bureau (No. Science and Technology Talents of Aunyi City 2021 No. 4), National Key R & D Plan for Research on Modernization of Traditional Chinese Medicine (No. 2017YFC1702005), Post Subsidy Project of State Key R & D Plan in Social Development Field (No. SQ2017YFC170204-05), the “hundred” level of high-level innovative talents in Guizhou Province (No. QKHRCPT 20165684), Program for Changjiang Scholars and Innovative Research Team in University, China (No. IRT_17R113), and Program for Outstanding Youth of Zunyi Medical University (No. 15zy-002).

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