Effect of different plant growth regulators on callus and adventitious shoots induction, polysaccharides accumulation and antioxidant activity of Rhodiola dumulosa

doi:https://doi.org/10.1016/j.chmed.2022.07.005

Archive > Volume 15 Issue 2 > 2023,15(2):271-277. DOI:https://doi.org/10.1016/j.chmed.2022.07.005 Prev Next

不同植物生長調(diào)節(jié)劑對(duì)小叢紅景天愈傷組織、不定芽誘導(dǎo)、多糖積累及抗氧化活性的影響

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Effect of different plant growth regulators on callus and adventitious shoots induction, polysaccharides accumulation and antioxidant activity of Rhodiola dumulosa

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摘要:

小叢紅景天（Rhodiola dumulosa (Franch.) S. H. Fu）是一種藥用植物，但其資源匱乏，為有效保護(hù)和利用這一資源，本研究以其莖段為外植體，分析了幾種不同植物生長調(diào)節(jié)劑（PGRs）對(duì)愈傷組織誘導(dǎo)、不定芽分化的影響。研究結(jié)果表明，MS培養(yǎng)基添加BA 0.85 mg/L、NAA 0.34 mg/L和2,4-D 0.33 mg/L是誘導(dǎo)愈傷組織的適宜培養(yǎng)基，誘導(dǎo)率可達(dá)90.03%。MS+TDZ 0.5 mg/L + NAA 2.0 mg/L培養(yǎng)基有利于愈傷組織的增殖，鮮重增加47.26倍，MS+BA 1.0 mg/L+NAA 0.5 mg/L培養(yǎng)基可誘導(dǎo)不定芽，誘導(dǎo)率為40.00%。MS+IBA1.0 mg/L的生根率最高，每芽可生10-15條根，長度為2-3 cm。此外，MS+ TDZ 0.5 mg/L+ NAA 2.0 mg/L、MS+BA 1.0 mg/L + NAA 0.5 mg/L培養(yǎng)基誘導(dǎo)的愈傷組織多糖含量高達(dá)1.72%?2.15%。在MS+NAA2.0 mg/L+TDZ 0.5 mg/L 、MS+BA 1.0 mg/L+NAA0.5和MS+BA 0.5 mg/L+2,4-D 0.5 mg/L培養(yǎng)基誘導(dǎo)的愈傷組織中多糖提取物對(duì)ABTS自由基消除率分別為82.78%、80.18%和68.59%，遠(yuǎn)高于野生植株。本研究建立了小叢紅景天的快速繁殖體系，并發(fā)現(xiàn)TDZ和NAA或BA和NAA的組合可以提高總多糖的產(chǎn)量，具有較強(qiáng)的自由基清除活性，為其資源的保護(hù)和利用奠定了基礎(chǔ)。

Abstract:

Objective: As a medicinal plant, the resource of Rhodiola dumulosa is deficient along with the large collection. For the protection and utilization of R. dumulosa, the influence of plant growth regulators (PGRs) on callus induction and adventitious shoots differentiation, polysaccharide production and the antioxidant activity were tested. Methods: Internodes of R. dumulosa were used as explants and cultured on MS medium plus different plant growth regulators (PGRs). The anti-oxidative activities of polysaccharides were evaluated using radical scavenging assays. Results: By response surface plot, 0.85 mg/L N6-benzyladenine (BA), 0.34 mg/L naphthaleneacetic acid (NAA) and 0.33 mg/L 2,4-dicholorophenoxyacetic acid (2,4-D) were the optimal factors for callus induction (90.03%) from internodes explants on MS medium. The fresh weight of green callus increased 47.26 fold, when callus was inoculated on MS + thidiazuron (TDZ) 0.5 mg/L + NAA 2.0 mg/L. Adventitious buds regenerated from callus on the media of MS were fortified with BA 1.0 mg/L plus NAA 0.5 mg/L, and the induction rate was 40.00%. MS plus indole-3-butyric acid (IBA) 1.0 mg/L produced the highest rooting rate with 10 to 15 roots in a length of 2–3 cm per shoot. The content of total polysaccharides in callus developed on MS + TDZ 0.5 mg/L + NAA 2.0 mg/L and MS + BA 1.0 mg/L + NAA 0.5 mg/L was as high as 1.72%– 2.15%. At the dose of 0.5 mg/mL polysaccharides extracted from different callus induced on MS + NAA 2.0 mg/L + TDZ 0.5 mg/L or MS + BA 1.0 mg/L + NAA 0.5 mg/L or MS + BA 0.5 mg/L + 2,4-D 0.5 mg/L, the ABTS radical eliminating percentages were 82.78%, 80.18% and 68.59%, respectively, much higher than that of wild plant. Conclusion: A rapid micropropagation system for R. dumulosa has been developed. The combination of TDZ and NAA or BA and NAA can increase the yield of the total polysaccharides. The polysaccharides isolated from callus and whole wild plants had stronger free radicals scavenging activities, indicating that polysaccharides from R. dumulosa are the potential pharmaceutical supplements.

關(guān)鍵詞:

不定芽;多糖;自由基清除;小叢紅景天

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This study was financially supported by the Independent Research Project of Graduate Students (No. BZKY2021035).

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