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Archive > Volume 15 Issue 2 > 2023,15(2):278-283. DOI:https://doi.org/10.1016/j.chmed.2022.11.003 Prev Next

基于葉綠體DNA揭示中國亞熱帶青風(fēng)藤遺傳種群結(jié)構(gòu)

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Chloroplast DNA reveals genetic population structure in Sinomenium acutum in subtropical China

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    摘要:
    目的：近年來，因藥用價(jià)值導(dǎo)致青風(fēng)藤過度采挖，大大降低了青藤的種群密度和多樣性。因此，迫切需要調(diào)查剩余種群，并制定利用和保護(hù)該物種的戰(zhàn)略。本研究旨在尋找可能的冰川避難所，并確定青風(fēng)藤的遺傳多樣性，以便其合理利用和保護(hù)。 方法：在中國亞熱帶的秦嶺、大巴山、大婁山和雪峰山四個(gè)山脈共采集了77個(gè)青風(fēng)藤樣本。使用四種葉綠體DNA分子標(biāo)記（atpI-atpH、trnQ-5'rps16、trnH-psbA和trnL-trnF）對(duì)這些群體之間的遺傳多樣性進(jìn)行了系統(tǒng)發(fā)育分析。 結(jié)果：在收集的樣本中共發(fā)現(xiàn)14個(gè)單倍型（C1至C14）。單倍型C1和C3在所有群體中共享，C3是祖先單倍型。單倍型C11和C12與C3和其他單倍型的差異最大。使用GST/NST測(cè)試，在四個(gè)位置沒有發(fā)現(xiàn)明顯的系統(tǒng)地理結(jié)構(gòu)。根據(jù)錯(cuò)配分布，以及Tajima的D檢驗(yàn)和Fu的FS檢驗(yàn)結(jié)果，沒有證據(jù)表明青風(fēng)藤種群快速擴(kuò)張。分子變異的分析揭示了群體內(nèi)的高度遺傳變異。相比之下，青風(fēng)藤種群之間的遺傳分化較低，表明基因流動(dòng)頻繁。 結(jié)論：雪峰山、大婁山和大巴山可能是青風(fēng)藤種群的冰川避難所。青風(fēng)藤的C1、C3、C11和C12單倍型因其重要的遺傳價(jià)值應(yīng)仔細(xì)保存和管理。

    Abstract:
    Objective: The population density and diversity of Sinomenium acutum (Menispermaceae) have been greatly reduced recently by overharvesting for medicinal purposes in China. Therefore, it is urgent that the remaining populations are investigated, and that strategies for the utilization and conservation of this species are developed. This study aimed to find the possible glacial refugia and define the genetic diversity of S. acutum for its proper utilization and conservation. Methods: A total of 77 S. acutum samples were collected from four locations, Qinling Mountains, Daba Mountains, Dalou Mountains, and Xuefeng Mountains, in subtropical China. Genetic diversity among and between these populations were phylogenetically analyzed using four chloroplast DNA molecular markers (atpI-atpH, trnQ-50 rps16, trnH-psbA and trnL-trnF). Results: A total of 14 haplotypes (C1 to C14) were found in collected samples. Haplotypes C1 and C3 were shared among all populations, with C3 as the ancestral haplotype. Haplotypes C11 and C12 diverged the most from C3 and other haplotypes. No obvious phylogeographic structure was found in four locations using the GST/NST test. There is no evidence of rapid demographic expansion in S. acutum based on the mismatch distribution, and the results of Tajima’s D test, and Fu’s FS test. Our analyses of molecular variance revealed a high level of genetic variation within populations. In contrast, the genetic differentiation among S. acutum populations was low, indicating frequent gene flow. Conclusion: Xuefeng, Dalou, and Daba Mountains were possible glacial refugia for the populations of S. acutum. C1, C3, C11 and C12 haplotypes of S. acutum should be carefully preserved and managed for their genetic value.

    關(guān)鍵詞:
    葉綠體DNA;遺傳多樣性;系統(tǒng)發(fā)育;種群變異;青風(fēng)藤

    Keywords:


    Project Supported:
    The authors would like to thank Jinjing Teng, Tao Guo, Yuan Chen, Shuaifu Li (College of Bioscience and Biotechnology, Hunan Agricultural University) for their help in experiment and professor Zhanguo Xin and Dr Huang Li for the revision of the paper. This study was supported by the Hunan Provincial Major Science and Technology Project (2015SK1001), Changsha Science and Technology Plan (kq1801015), Project of Hunan Administration of Traditional Chinese Medicine (2021212), Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine (HCSW2020-01).

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