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Archive > Volume 15 Issue 2 > 2023,15(2):298-309. DOI:https://doi.org/10.1016/j.chmed.2022.11.002 Prev Next

轉(zhuǎn)錄組學(xué)和代謝組學(xué)相結(jié)合的分析揭示了金線蘭不同器官合成二萜類化合物的復(fù)雜代謝基因

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Combined analysis of transcriptomics and metabolomics revealed complex metabolic genes for diterpenoids biosynthesis in different organs of Anoectochilus roxburghii

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    摘要:
    目的：金線蘭是一種珍貴的藥用植物，其重要的活性成分是富含多種生物活性的二萜類化合物。然而，由于缺乏金線蘭中二萜代謝過(guò)程的遺傳信息，參與二萜代謝分子調(diào)控機(jī)制的基因尚不清楚。本研究通過(guò)轉(zhuǎn)錄組學(xué)和代謝組學(xué)相結(jié)合的方法，揭示了金線蘭不同器官合成二萜類化合物的復(fù)雜代謝基因。 方法：采用代謝組學(xué)方法分析金線蘭根、莖、葉中二萜積累的差異，并通過(guò)轉(zhuǎn)錄組測(cè)序獲得其代謝基因信息。然后，從基因表達(dá)模式的角度分析了金線蘭不同器官中二萜類化合物差異積累的分子機(jī)制。 結(jié)果：在金線蘭5種萜類代謝途徑中共鑒定出296種萜類代謝產(chǎn)物。根與葉、葉與莖和根與莖之間分別有38種、34種和18種不同含量的二萜類化合物。鑒定出參與二萜合成過(guò)程的29個(gè)代謝酶基因，共883個(gè)單基因，預(yù)測(cè)萜類主干生物合成階段的DXS和FDPS以及二萜生物合成階段的CPA、GA20ox、GA3ox、GA20ox和MAS是二萜積累的關(guān)鍵代謝酶。此外，預(yù)計(jì)有14個(gè)關(guān)鍵轉(zhuǎn)錄因子編碼基因參與了二萜生物合成的調(diào)控。GA20ox、MAS、CPA、GA20ox和GA3ox等基因的表達(dá)可能被14個(gè)轉(zhuǎn)錄因子中的一些激活。預(yù)測(cè)轉(zhuǎn)錄因子NTF-Y和PRE6是最重要的轉(zhuǎn)錄因子。 結(jié)論：本研究確定了金線蘭中29個(gè)代謝酶基因，預(yù)測(cè)了14個(gè)參與二萜代謝分子調(diào)控機(jī)制的轉(zhuǎn)錄因子，為進(jìn)一步研究金線蘭不同器官中二萜類物質(zhì)積累的分子調(diào)控機(jī)制提供了參考。

    Abstract:
    Objective: Diterpenoids with a wide variety of biological activities from Anoectochilus roxburghii, a precious medicinal plant, are important active components. However, due to the lack of genetic information on the metabolic process of diterpenoids in A. roxburghii, the genes involved in the molecular regulation mechanism of diterpenoid metabolism are still unclear. This study revealed the complex metabolic genes for diterpenoids biosynthesis in different organs of A. roxburghii by combining analysis of transcriptomics and metabolomics. Methods: The differences in diterpenoid accumulation in roots, stems and leaves of A. roxburghii were analyzed by metabonomic analysis, and its metabolic gene information was obtained by transcriptome sequencing. Then, the molecular mechanism of differential diterpenoid accumulation in different organs of A. roxburghii was analyzed from the perspective of gene expression patterns. Results: A total of 296 terpenoid metabolites were identified in the five terpenoid metabolic pathways in A. roxburghii. There were 38, 34, and 18 diterpenoids with different contents between roots and leaves, between leaves and stems, and between roots and stems, respectively. Twenty-nine metabolic enzyme genes with 883 unigenes in the diterpenoid synthesis process were identified, and the DXS and FDPS in the terpenoid backbone biosynthesis stage and CPA, GA20ox, GA3ox, GA2ox, and MAS in the diterpenoid biosynthesis stage were predicted to be the key metabolic enzymes for the accumulation of diterpenoids. In addition, 14 key transcription factor coding genes were predicted to be involved in the regulation of the diterpenoid biosynthesis. The expression of genes such as GA2ox, MAS, CPA, GA20ox and GA3ox might be activated by some of the 14 transcription factors. The transcription factor NTF-Y and PRE6 were predicted to be the most important transcription factors. Conclusion: This study determined 29 metabolic enzyme genes and predicted 14 transcription factors involved in the molecular regulation mechanism of diterpenoid metabolism in A. roxburghii, which provided a reference for the further study of the molecular regulation mechanism of the accumulation of diterpenoids in different organs of A. roxburghii.

    關(guān)鍵詞:
    金線蘭;二萜代謝途徑;葉子;代謝組學(xué);根;莖;轉(zhuǎn)錄組

    Keywords:


    Project Supported:
    This work was financially supported by grants from the Department of Technology and Science of the Fujian Provincial Government (No. 2021J01132; No. 2020J01732), and the Fujian Agriculture and Forestry University Science and Technology Innovation Special Fund (No. KFA18071A).

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