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Archive > Volume 16 Issue 1 > 2024,16(1):143-150. DOI:https://doi.org/10.1016/j.chmed.2023.01.003 Prev Next

基于DNA宏條形碼技術(shù)的四種根類藥材表面真菌群落研究

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DNA metabarcoding analysis of fungal community on surface of four root herbs

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    摘要:
    目的：全世界普遍將當歸、肉蓯蓉、人參和西洋參加工成藥食兩用的補品，但這四種藥材極易被真菌和真菌毒素污染。因此，本研究將使用DNA宏條形碼技術(shù)分析它們表面真菌群落的組成。 方法：本研究從中國四大主產(chǎn)區(qū)共采集到12個根類藥材樣品，這些樣品根據(jù)藥材的種類分為四組，分別是ASR組、CH組、PG組和PQ組。通過DNA宏條形碼技術(shù)定位識別四種根類藥材表面真菌群落的ITS2序列，從而對其進行分析。 結(jié)果：12個樣品均檢測到真菌感染，其中根霉屬 (13.04%?74.03%)、曲霉屬(1.76%?23.92%)和鐮刀菌屬(0.26%?15.27%)占比最高。在種水平上，本研究共檢測到10種真菌，包括2個潛在產(chǎn)毒真菌，分別是橘青霉和草酸青霉，以及8個人類致病真菌，分別是Alternaria infectoria、Candida sake、Hyphopichia burtonii、Malassezia globosa、M. restricta、Rhizopus arrhizus、Rhodotorula mucilaginosa和Ochroconis tshawytschae。ASR組和CH組的真菌群落組成與其他組的真菌群落組成存在顯著性差異，而PQ組真菌群落組成與PG組真菌群落組成相似。 結(jié)論：DNA宏條形碼技術(shù)揭示了四種重要根類藥材表面真菌群落的組成情況，本研究為防止根類藥材免受真菌和真菌毒素污染提供了重要參考依據(jù)。

    Abstract:
    Objective: Angelicae Sinensis Radix (ASR, Danggui in Chinese), Cistanches Herba (CH, Roucongrong in Chinese), Ginseng Radix et Rhizoma (PG, Renshen in Chinese), and Panacis Quinquefolii Radix (PQ, Xiyangshen in Chinese), widely used as medicine and dietary supplement around the world, are susceptible to fungal and mycotoxin contamination. In this study, we aim to analyze their fungal community by DNA metabarcoding. Methods: A total of 12 root samples were collected from three main production areas in China. The samples were divided into four groups based on herb species, including ASR, CH, PG, and PQ groups. The fungal community on the surface of four root groups was investigated through DNA metabarcoding via targeting the internal transcribed spacer 2 region (ITS2). Results: All the 12 samples were detected with fungal contamination. Rhizopus (13.04%-74.03%), Aspergillus (1.76%-23.92%), and Fusarium (0.26%-15.27%) were the predominant genera. Ten important fungi were identified at the species level, including two potential toxigenic fungi (Penicillium citrinum and P. oxalicum) and eight human pathogenic fungi (Alternaria infectoria, Candida sake, Hyphopichia burtonii, Malassezia globosa, M. restricta, Rhizopus arrhizus, Rhodotorula mucilaginosa, and Ochroconis tshawytschae). Fungal community in ASR and CH groups was significantly different from other groups, while fungal community in PG and PQ groups was relatively similar. Conclusion: DNA metabarcoding revealed the fungal community in four important root herbs. This study provided an important reference for preventing root herbs against fungal and mycotoxin contamination.

    關(guān)鍵詞:
    當歸;肉蓯蓉;DNA宏條形碼技術(shù);真菌;人參;ITS2;西洋參;根類藥材

    Keywords:


    Project Supported:
    This work was supported by CAMS Innovation Fund for Medical Sciences (CIFMS) (No. 2021-I2M-1-071).

    Clc Number:

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