醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

Archive > Volume 16 Issue 2 > 2024,16(2):293-300 Prev Next

含馬兜鈴酸草藥中主要活性/毒性成分含量分析

Original articles

Chemical profiling of principle active and toxic constituents in herbs containing aristolochic acids

  • Article
  • Figures
  • Metrics
  • Preview PDF
  • Reference
  • Related
  • Cited by
  • Materials
    摘要:
    目的：含馬兜鈴酸草藥（HCAAs）在中國(guó)許多少數(shù)民族地區(qū)仍被用做藥物和/或調(diào)味料，本研究旨在明確HCAAs中主要活性/毒性成分的含量。 方法：本研究中，以6-O-甲基鳥(niǎo)苷為內(nèi)標(biāo)，運(yùn)用超高效液相色譜-電噴霧離子源-串級(jí)質(zhì)譜（UPLC-ESI-MS/MS）對(duì)超聲提取的HCAAs中六種主要活性/毒性成分進(jìn)行定性定量分析。該方法采用了多反應(yīng)監(jiān)測(cè)-信息依賴(lài)采集-增強(qiáng)子離子掃描模式（MRM-IDA-EPI），并結(jié)合了動(dòng)態(tài)背景扣除（DBS）。 結(jié)果：6種目標(biāo)化合物在0.01- 0.27 ng/mL的濃度范圍內(nèi)呈現(xiàn)出良好線(xiàn)性。提取重復(fù)性、方法精密度、方法準(zhǔn)確性都很好。提取液在15 °C條件下可穩(wěn)定保存24小時(shí)。除了2號(hào)樣品中未檢出馬兜鈴內(nèi)酰胺之外，其余樣品中6種目標(biāo)化合物都有檢出，且含量結(jié)果差別較大。山草果果實(shí)、葉、莖中目標(biāo)化合物的含量依次降低。 結(jié)論：該研究中建立的分析方法具有樣品量小，操作簡(jiǎn)單，分析時(shí)間短，高靈敏度、高特異性和高準(zhǔn)確性等特點(diǎn)。為HCAAs 的安全使用，藥效毒效的深入研究，以及科學(xué)炮制和配伍奠定了良好基礎(chǔ)。

    Abstract:
    Objective: To clear the amounts of the principal active/toxic components in herbs containing aristolochic acids (HCAAs), which are still used as medicine and/or seasoning in many ethnic minority areas of China. Methods: In this study, six major active and toxic components in HCAAs were extracted with ultrasonic extraction. With 6-O-methyl guanosine as internal standard, the target compounds were analyzed qualitatively and quantitatively by using ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) with multiple reaction monitoringinformation dependent acquisition-enhanced production ion scanning mode (MRM-IDA-EPI) combined with dynamic background subtraction (DBS) function. Results: The method showed good linearity in the linear range of the six analytes. The limit range of detection was from 0.01 ng/mL to 0.27 ng/mL. All of the detection repeatability, extraction repeatability and accuracy of the method were good. After extraction, the samples remained stable at 15℃ within 24 h. Six analytes were all found in samples except aristolactam (AL) in sample 2, and the contents varied greatly. The contents of these compounds decreased in fruits, leaves and stems of Aristolochia delavayi successively. Conclusion: This method has the advantages of less sample dosage, simple operation, short analysis cycle, high sensitivity, specificity and accuracy. It laid a good foundation for guiding the safety of HCAAs, the indepth study of pharmacological and toxicological effects and the scientific and standardized processing and compatibility of HCAAs.

    關(guān)鍵詞:
    馬兜鈴酸;馬兜鈴內(nèi)酰胺;化學(xué)分析;含馬兜鈴酸草藥;超高效液相色譜-電噴霧離子源-串級(jí)質(zhì)譜(UPLC-ESI-MS/MS)

    Keywords:


    Project Supported:
    This study was supported by the National Natural Science Foundation of China (No. 81603076), and Minzu University of China (No. 2021MDYY53).

    Clc Number:

WeChat

Mobile website