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Archive > Volume 17 Issue 2 > 2025,17(2):315-321. DOI:https://doi.org/10.1016/j.chmed.2025.02.001 Prev Next

通過板頭香不同分層真菌組成揭示沉香部位含有黃曲霉抑制物

Original articles

Analysis of fungal composition in different layers of Bantou agarwoodforming trunk of Aquilaria sinensis revealing presence of Aspergillusinhibiting substances in agarwood sites

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    摘要:
    目的:研究目的是分析板頭香真菌組成及板頭香的開發(fā)應用潛力。方法:板頭香是白木香樹被砍傷后自然形成的沉香。采用CTAB法提取板頭香真菌的基因組總DNA，進行PCR擴增和文庫構建。通過HiSeq2500平臺獲得有效序列，并對數(shù)據(jù)進行生物信息學和統(tǒng)計學分析。結果:共獲得有效序列7850,040條，在門的水平上，子囊菌門真菌豐度最高，相對豐度為56.36 ~ 61.44%，擔子菌門次之，相對豐度為10.49 ~ 20.39%。Dothideomyetes、Agaricomyetes和Sordariomyetes。在綱的水平上占優(yōu)勢，分別占26.21 ~ 33.88%、8.40 ~ 17.66%和18.41 ~ 24.11%。在屬的水平上以Lignosphaeria、Phaeoacremonium和Hermatomyces為優(yōu)勢菌，相對豐度分別為6.25 ~ 7.64%、1.95 ~ 9.05%和1.5 ~ 5.4%。多樣性和豐富度分析表明，沉香形成部位(沉香層、沉香過渡上層和沉香過渡下層)的真菌組成顯著低于腐爛層和健康層。也就是說，在沉香形成過程中，真菌的多樣性和豐富度在砍傷開放性傷口的作用下顯著降低。沉香腐爛層和白木層與沉香形成部位的真菌群落結構明顯不同。沉香形成部位曲霉類群數(shù)量顯著減少 (健康層為0.5%，腐爛層為0.022%，沉香過渡上層為0.012%，沉香層為0.01%，沉香過渡下層為0.013%)，說明沉香中可能含有抑制曲霉生長的潛在物質(zhì)。結論:沉香含有抑制曲霉的潛在物質(zhì)，為曲霉屬的防治提供了有價值的信息。

    Abstract:
    Objective: The objective of this study was to analyse fungal composition and exploit application potential in the Bantou (BT) agarwood-forming trunk of Aquilaria sinensis. Methods: BT agarwood is a naturally formed agarwood that was collected after cutting. Total genomic DNA of the fungi in BT agarwood was extracted by the hexadecyltrimethy ammonium bromide (CTAB) method, followed by PCR amplification and library construction. The effective tags were obtained by the HiSeq2500 platform, and the data were subjected to bioinformatics and statistical analyses. Results: A total of 7 850 040 effective tags were obtained, Ascomycota was the most abundant fungus at the phylum level, with a relative abundance of 56.36%–61.44%, followed by Basidiomycota, with a relative abundance of 10.49%–20.39%. Dothideomycetes, Agaricomycetes and Sordariomycetes were dominant at the class level, accounting for 26.21%–33.88%, 8.40%–17.66%, and 18.41%–24.11%, respectively. Lignosphaeria, Phaeoacremonium and Hermatomyces were dominant at the genus level, with relative abundances of 6.25%–7.64%, 1.95%–9.05% and 1.5%–5.4%, respectively. Diversity and richness analysis showed that the fungal composition in the agarwood formation sites (agarwood layer, upper agarwood layer and lower agarwood layer) were significantly lower than those in the decomposing layer and the healthy layer. That is, the fungal diversity and richness were significantly reduced during agarwood formation by the action of open wounds. The fungal community structure in the decomposing layer and agarwood formation sites obviously differed from that in the healthy layer. The number of Aspergillus taxa in agarwood formation sites decreased significantly (healthy layer is 0.5%, decomposing layer is 0.022%, upper agarwood layer is 0.012%, agarwood layer is 0.01%, and lower agarwood layer is 0.013%), indicating that agarwood may contain potential substances to inhibit the growth of Aspergillus. Conclusion: Agarwood from agarwood formation sites contains potential substances that inhibit Aspergillus, which provides valuable information for the control of the genus of Aspergillus.

    關鍵詞:
    曲霉菌;;沉香;板頭香;真菌組成

    Keywords:


    Project Supported:
    This study was funded by the National Key Research and Development (No. 2022YFC3501504); the Key Project of Research and Development Project of Hainan Province (No. ZDYF2022SHFZ035) and the CAMS Innovation Fund for Medical Sciences (CIFMS) (No. 2021-I2M-1-032).

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