目的 研究北冬蟲夏草菌絲體醇提物（EECM）對氫化可的松致小鼠腎損作的保護(hù)作用，并對其可能的作用機(jī)制進(jìn)行探討。方法 ICR雄性老齡小鼠隨機(jī)分為正常組，模型組，EECM低、中、高劑量［0.167、0.334、0.668 g/(kg?d）］組和參仙壯腎膠囊［0.250 g/(kg?d）］組。除正常組和模型組分別給以生理鹽水外，其他各組每日一次給以相應(yīng)的藥物，連給30 d；除正常組外，在每次給藥后1 h均im氫化可的松25 mg/(kg?d)造模，連給7 d。最后一次給藥結(jié)束后測定小鼠血清尿素氮（BUN）、肌酐（Scr）、促腎上腺皮質(zhì)激素（ACTH）和皮質(zhì)醇（Cor）的水平，并通過光鏡觀察小鼠腎組織病理變化。結(jié)果 與模型組相比，EECM可顯著降低模型小鼠腎臟系數(shù)、BUN和Scr的量；提高小鼠體質(zhì)量、血清ACTH和Cor水平；減輕氫化可的松引起的腎臟組織病理損害。結(jié)論 EECM對氫化可的松致老齡小鼠腎功能損害具有腎保護(hù)作用。

Objective To study the effect of ethanol extract of Cordyceps militaris mycelia (EECM) on hydrocortisoneinduced renal injury in aging mice and the possible mechanisms. Methods Aging male ICR mice were randomly divided into 6 groups, normal guoup, model group, EECM ［0.167、0.334、0.668 g/(kg?d）］ groups, and Shenxian Zhuangshen Capsula ［0.250 g/(kg?d）］ group. All animals except thenormal control and model groups (were given equivalent amount of 0.85% sodium chloride solution) were administrated corresponding drugs once every day, and 30 d continuantly. All groups except the normal control the mice were injected with hydrocortisone 25 mg/(kg?d) at 1 h after administration, and for 7 d. After administration finally, the contents of BUN, Scr, ACTH, Cor in the serum were measured, and the kidney histological changes were observed by optical micorscope. Results Compared with to the model group, the contents of BUN, Scr in the serum, and the coefficent of kidney were obviously decreased in EECM groups； the contents of ACTH and Cor in the serum, and the avoirdupois of mice were obviously increased. Furthermore, EECM ameliorated the kidney pathological changes.Conclusion EECM has protective effects on hydrocortisoneinduced renal lesions in aging mice.

浙江省重大國際科技合作項(xiàng)目（2004C14009）