目的 建立同時測定金銀花及金芪降糖片中綠原酸的反相高效液相色譜法。方法 色譜柱為Diamondsil C18（250 mm×4.6 mm，5 μm），以乙腈0.4%磷酸溶液（13∶87）為流動相，檢測波長為327 nm，體積流量為1.0 mL/min。結(jié)果 在上述條件下測定金銀花中綠原酸為2.49%，金芪降糖片中綠原酸為8.776 mg/片。結(jié)論 本方法操作簡單、結(jié)果準確、回收率高、重現(xiàn)性好，可用于金銀花及金芪降糖片的質(zhì)量控制。

Objective To establish a reversedphase HPLC（RP-HPLC）method of determination of chlorogenic acid in Flos lonicerae and Jinqi Jiangtang Tablet. Methods To use RPHPLC for determination. The separation was carried out using the Diamosil C18 column (250 mm×4.6 mm，5 μm) with the mobile phase containing acetonitrile0.4% orthophosphoric acid (13∶87) and a detective wavelength at 327 nm. The flow rate was 1.0mL?min1. ResultsThe contents chlorogenic acid in Flos lonicerae and Jinqi Jiangtang Tablet were 2.49% and 8.776mg/t respectively. Conclusion The method is simple, accurate with a good recovery and repeatability. The method can be used to control the quality of Flos lonicerae and Jinqi Jiangtang Tablet.

天津市科技攻關(guān)項目（06YFGPSH02900）