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[摘要]
目的 為建立莫邪菊的離體培養(yǎng)體系。方法 以成熟果實中的種子為外植體,探討不同生長調節(jié)劑對離體培養(yǎng)中愈傷組織誘導;不定芽的分化和試管苗生根的影響。結果 培養(yǎng)基配方為MS+2,4-D 2.0 mg/L+6-BA 0.2 mg/L時,種子破殼萌發(fā)率為70%,胚軸處愈傷組織誘導率為100%;MS+6-BA 2.0 mg/L為合適的叢生芽誘導和增殖培養(yǎng)基配方;培養(yǎng)基為MS0時生根率為95%,生根效果好且配方簡單,移栽的試管苗成活率可達95%。結論 該試驗建立的離體培養(yǎng)體系可用于莫邪菊的離體培養(yǎng)。
[Key word]
[Abstract]
Pbjective To establish as in vitro eulture system for Carpobrotus edulis L. Methods Using C. edulis L. seeds in ripe fruits as explants, explore the effects of different plant growth regulators on callus induction, adventitious buds differentiation, and rooting in vitro. Results When the media was MS+2,4-D 2.0 mg/L+6-BA 0.2 mg/L, the germination rate was 70%, and callus induction rate was 100%. The optimum media for caespitose buds induction and proliferation was MS+6-BA 2.0mg/L. Both plantlets rooting rate and survival rate reached 95% when cultured on simple medium MS0. Conclusion The established in vitro culture system can be used for C. edulisL.
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