目的 建立心肌梗死超急性期心肌缺血模型。方法 用清醒比格犬與遙控實時檢測技術(shù)，測定冠脈前降支氣囊壓迫狹窄后心肌缺血的電生理特征與血壓變化。結(jié)果 隨冠脈流量的下降，ST段電壓先出現(xiàn)壓低(壓低相)，再呈現(xiàn)電壓幅度升高(緩臺相、急抬相），并伴隨T波、R波電壓不同程度地升高；ST段、T波、R波電壓在不同時相變化率不同，提示其敏感性不同，且T波、R波電壓在不同時相與ST段電壓變化相關(guān)性不同；Q波電壓與時程，以及心率(HR)、血壓(BP)無明顯變化。結(jié)論 采用清醒比格犬與遙控實時檢測技術(shù)建立的心肌梗死超急性期缺血模型，其特點與臨床一致，方法科學(xué)、可靠。

Objective To establish the ischemia model for hyperacute-phase myocardial infarction. Methods Conscious Beagle dogs were chosen as the experimental animals. Remote real-time detection technology was applied to measure the electrophysiological characteristics of myocardial ischemia and changes in blood pressure after anterior descending coronary artery stenosis with balloon tamponade. Results With the decline in coronary blood flow, voltage of ST segment was depressed firstly (depressed stage),and then elevated (slow lift stage, acute lift stage), accompanied with T and R wave voltage increasing in different degrees. The variation rates of ST, T and R-wave voltage were various in different stage, suggesting a characteristic sensitivity of electrophysiological wave. In addition, there was diference in the relevance of change between T or R-wave and ST segment in different stage. However, in this model, there was no significant change in Q-wave voltage, heart rate and blood pressure. Conclusion It is scientific and reliable to use conscious Beagle dogs and remote real-time detection technology to establish the ischemia model for hyperacute-phase myocardial infarction.

國家科技支撐計劃項目(NO.2007BAI41B02)；系列化、國際化的國家生物醫(yī)藥國際創(chuàng)新園新藥研發(fā)綜合性大平臺建設(shè)項目(2009ZX09301-008)