目的 研究蛇床子鎮(zhèn)靜催眠作用的有效性、宿醉反應和耐受性。方法 觀察蛇床子生藥、醇提物對小鼠自主活動和對戊巴比妥鈉催眠劑量潛伏期和睡眠時間的影響；觀察蛇床子醇提物對小鼠醒后自主活動的影響，及其重復給藥對自主活動和催眠實驗的影響。結果 蛇床子生藥、65%、95%醇提物組小鼠自主活動次數(shù)顯著低于空白對照組，且睡眠時間均顯著長于空白對照組；地西泮、65%醇提取物小鼠睡前站立次數(shù)顯著少于空白對照組；65%、95%醇提物組小鼠醒后活動次數(shù)均顯著高于空白對照組；蛇床子生藥、65%、95%醇提物組小鼠于給藥第1、3、5、15天站立次數(shù)均少于空白對照組。對自主活動抑制作用是逐漸加強的，二地西泮對自主活動均數(shù)的影響在給藥后的前5 d逐漸減少，第15天次數(shù)增加。蛇床子各給藥組睡眠時間均數(shù)隨用藥時間的延長而增加，而地西泮則反之。結論 蛇床子鎮(zhèn)靜催眠作用顯著，且宿醉反應和耐受性等較地西泮小。

Objective To study the efficacy, tolerance and hangover response of sedation and hypnosis of ethanol extract of Fructus Cnidii on mice. Methods Crude drug and ethanol of Fructus Cnidii were studied the effects on autonomic activities, and dose, latent period and duration of hypnosis sleep induced by sodium pentobarbital injection. The ethanol extracts were also studies the autonomic activities after sleep. The effects of repeated-dose on autonomic avtivities and hypnosis were also studied. Results Compared with the blank control group, the times of autonomic activities in groups of crude drug, 65% ethanol extract and 95% ethanol extract were significantly lower, and the sleep time in the groups of crude drug and ethanol extract was much longer. The frequencies of pre-sleep standing in diazepam group and 65% ethanol extract were significantly lower. The times of activities after sleep in the two ethanol extract groups were obviously more, and the times of standing on 1, 3, 5, 15 day after administration of crude drug, ethanol extracts were obviously less. The sleep time in experimental groups increased with the time after Fructus Cnidii administration, while decreased with diazepam administration. Fructus Cnidii ethanol extracts did not showed drug tolerance, while diazepam did. Conclusion Fructus Cnidii has a significant sedative and hypontic effects on mice, and the hangover response and tolerance are less than diazepam.

山西省衛(wèi)生廳科技攻關項目(200515)