目的 提高二母安嗽丸的質量控制標準。方法 采用薄層色譜法對兒母安嗽丸中的知母、玄參、罌粟殼、款冬花、紫苑、苦杏仁進行定性鑒別，采用高效液相色譜法測定二母安嗽丸中嗎啡的量。色譜柱為Dikma Diamonsil C₁₈柱(250 mm×4.6 mm,5μm)；流動相為乙腈-0.1%磷酸溶液(15:85)(每100 mL加0.1 g庚烷磺酸鈉)；柱溫為40 ℃；體積流量為1.0 mL/min；檢測波長為220 nm。結果 知母、玄參、罌粟殼、款冬花、紫苑、苦杏仁薄層色譜半點清晰，重現性好，無干擾；嗎啡在2.078～83.12 μg線性關系良好，平均回收率為96.18%，RSD為1.03%(n＝6)。結論 所建立的定性和定量方法結果準確、可靠，重現性好，可用于二母安嗽丸的質量控制。

Objective To improve the quality standard for Ermu Ansou Pill. Methods TLC was applied to identify Rhizoma Anemarrhenae, Radix Scrophulariae, Pericarpium Papaveris, Flos Farfarae, Radix Et Rhizoma Asteris and Semen Armeniacae Amarum, HPLC was applied to determine the content of morphine ine the medicine. Results The TLC spots were distinct and reproducible. The linear range of morphine was 2.078～83.12% μg/mL. The average recovery rate was 96.18%, ang RSD was 1.03% (n＝6). Conclusion The qualitative and quantitative methods established in this study are feasible, accurat and reproducible. The can be well used as the quality standard control for Ermu Ansou Pill.