目的 建立RP-HPLC法同時(shí)測(cè)定女貞葉乙醇提取物中齊墩果酸與熊果酸的方法。方法 通過(guò)系統(tǒng)地考察溫度、pH值、甲醇-乙腈混合有機(jī)相中甲醇的量對(duì)齊墩果酸、熊果酸保留時(shí)間和分離度的影響，最終確定HPLC色譜條件為大連依利特Kromasil C18（250 mm×4.6 mm，5 μm）,色譜柱；甲醇-乙腈-10 mmol/L乙酸銨(55∶27∶18)為流動(dòng)相，體積流量為1 mL/min，檢測(cè)波長(zhǎng)為205 nm，柱溫20 ℃。結(jié)果 齊墩果酸與熊果酸分別在0.300～300 μg、0.692～6.92 μg線性關(guān)系良好；加樣平均回收率分別為101.4%、100.2%(n=9)。結(jié)論 低溫、中性pH、甲醇-乙腈混合有機(jī)相有利于齊墩果酸與熊果酸的分離。優(yōu)化后的色譜方法分離度高，重現(xiàn)性好，可用于女貞葉乙醇提取物中齊墩果酸與熊果酸的測(cè)定。

Objective To establish a RPHPLC method for simultaneous quantitative determination of oleanolic acid and ursolic acid in ethanol extract of Ligustrum lucidum Aitleaves.Methods Temperature，pH value and proportion of methanol and acetonitrile in organic phase were systematically studied on the influence of retention and resolution between oleanolic acid and ursolic acid. The optimized chromatographic condition was Kromasil C18 column (250 mm×4.6 mm, 5 μm);methanolacetonitrile 10 mmol/L ammonium acetate (55∶27∶18) as mobile phase with flow rate 1.0 mL/min. The detection wavelength was 205 nm and col umn temperature was 20 ℃.Results The linear ranges of oleanolic acid and ursolic acid were 0.300～3.00 μg，0.692～6.92 μg respectively; the average recoveries were 101.4%， 100.2%(n=9) respectively.Conclusion Low temperature，neutral pH and mixed organic phase with methanolacetonitrile can improve resolution of oleanolic acid and ursolic acid. The optimized method can achieve better resolution and reproducibility，and is suitable to determine the content of oleanolic acid and ursolic acid in ethanol extract of Ligustrum lucidum Ait leaves.