[關(guān)鍵詞]
[摘要]
摘 要：目的 建立清醒比格犬的心衰模型。方法 比格犬分為模型組（將下腔靜脈直徑縮窄50%）和假手術(shù)組（僅在下腔靜脈處穿線不縮窄）。用清醒比格犬胸下腔靜脈縮窄法與遙控檢測技術(shù)，研究在心衰形成過程中的心臟功能與血流動(dòng)力學(xué)變化。結(jié)果 模型組動(dòng)物逐漸出現(xiàn)腹壁靜脈曲張、腹壁水腫、腹水等靜脈瘀血體征；術(shù)后 Tau 時(shí)間常數(shù)明顯延長，dp/dtmin 在4周內(nèi)增加，6周后開始降低；LVEDP 術(shù)后1～2周明顯降低（P＜0.001～0.01），術(shù)后5周開始升高，8～9周時(shí)升高更為顯著（P＜0.01～0.05）；術(shù)后6周 E 峰開始明顯下降，A 峰明顯升高，E/A 比值在術(shù)后即開始輕度減小，6～9周減小最明顯；手術(shù)后1～4周 dp/dtmax 明顯增加，6～9周的 dp/dtmax 明顯降低；術(shù)后8～9周 HR 增加（P＜0.05）；術(shù)后3周內(nèi) SBP 變化不明顯，4周后開始明顯增加，至9周時(shí)增加最為顯著；術(shù)后1周開始 DBP、MABP、LVSP 即明顯增加，持續(xù)至第9周。模型組術(shù)后耗氧量開始稍有增加，其中1、7周后與假手術(shù)組比較有明顯統(tǒng)計(jì)學(xué)差異（P＜0.05～0.01）。結(jié)論 采用清醒比格犬與遙控實(shí)時(shí)檢測技術(shù)建立的心衰模型，能模擬臨床心衰病人的部分特征，可用于抗心衰藥物研發(fā)與心衰疾病機(jī)制研究。
[Key word]
[Abstract]
Abstract: Objective To establish the model of heart failure by thoracic inferior vena caval constriction（TIVCC）in conscious Beagle dogs．Methods Conscious dogs were chosen as experiment animals．Telemetry monitoring technology was applied to measure the hemodynamic parameters，and the peaks of velocity at the tip of opened mitral valve leaflets were measured by echocardiography．Results Signs of varicosis，edema and hydroperitoneum appeared in conscious Beagle dogs with TIVCC．The Tau constant in model group was prolonged，dp/dtmin increased within 4 weeks after operation but declined at 6 weeks after operation，LVEDP reduced at 1 to 2 weeks while increased at 5 weeks．E wave decreased，A wave increased with decline of the ratio of E wave to A wave（E/A ratio）at 6 weeks after TIVCC．dp/dtmax increased from 1 to 4 weeks after operation，but decreased at 6 weeks．Heart rate increased significantly at 8 weeks，while DBP，MABP and LVSP increased at 1 week．The oxygen-consumption was increased，especially at 1 week and 7 weeks．Conclusion The model of heart failure established with real-time remote technology in conscious dogs was in accordance with clinical characteristics of patients with heart failure，and can be used in research and development of new agents for treatment and study on mechanism of pathogenesis in heart failure．
[中圖分類號]
[基金項(xiàng)目]
國家科技支撐計(jì)劃項(xiàng)目（2007BAI41B02）；系列化、國際化的國家生物醫(yī)藥國際創(chuàng)新園新藥研發(fā)綜合性大平臺建設(shè)項(xiàng)目（2009ZX09301-008）
