摘 要：目的 建立健兒消食口服液中橙皮苷和黃芩苷的定量測定方法。方法 采用 HPLC 法，色譜柱為 Agilent Eclipse XDB（250 mm × 4.6 mm，5 μm），流動(dòng)相為乙腈-0.3%磷酸溶液（23︰77），檢測波長283 nm。結(jié)果 橙皮苷在0.090 25～0.722 0 μg 呈良好的線性關(guān)系；黃芩苷在0.323 25～2.602 0 μg 呈良好的線性關(guān)系。平均回收率分別為99.62%、98.99%；RSD 分別為1.86%、1.66%。結(jié)論 本方法簡便、準(zhǔn)確、重現(xiàn)性好，可用于健兒消食口服液的質(zhì)量控制。

Abtract: Objective To establish a method for the simultaneous determination of hesperidin and baicalin in Jian′er Xiaoshi Oral Liquid．Methods RP-HPLC was adopted with Agilent Eclipse XDB（250 mm × 4.6 mm，5 μm）as analytical column．The mobile phase was acetonitrile - 0.3% phosphoric acid solution（23︰77）．The detection wavelength was at 283 nm．Results The linear range of hesperidin was 0.090 25—0.722 0 μg，the average recovery was 99.62% and RSD was 1.86%．The linear range of baicalin was 0.323 25—2.602 μg，the average recovery was 98.99%，and RSD was 1.66%．Conclusion The method is convenient，accurate and reproducible，and can be applied to quality control of Jian′er Xiaoshi Oral Liquid．