目的 研究仙鶴草醋酸乙酯有效部位（總鞣質質量分數(shù)為19.91%）對人肝癌HepG2細胞增殖的抑制作用及其機制。方法 以不同質量濃度的仙鶴草醋酸乙酯有效部位作用于體外培養(yǎng)的HepG2細胞，MTT法檢測細胞生長抑制率；流式細胞儀檢測細胞凋亡；Fluo-3/AM熒光探針觀察腫瘤細胞內(nèi)鈣離子濃度的變化；流式細胞儀檢測腫瘤細胞內(nèi)活性氧（ROS）的變化。結果 仙鶴草醋酸乙酯有效部位可顯著抑制HepG2細胞的生長，誘導細胞凋亡，其IC50為127.85 μg/mL。經(jīng)該有效部位作用48 h后，HepG2細胞數(shù)量明顯減少；在Fluo-3/AM熒光探針的作用下有較強的綠色熒光；同時檢測出細胞內(nèi)ROS有較明顯的增加。結論 仙鶴草醋酸乙酯有效部位能抑制HepG2細胞的生長，誘導細胞發(fā)生凋亡。腫瘤細胞內(nèi)鈣離子的釋放和細胞內(nèi)ROS的增加可能是其作用機制。

Objective To study the antiproliferation effects of extract from Agrimonia pilosa（tannin 19.91%）on hepatocellular carcinoma HepG2 cells and their mechanisms. Methods HepG2 cells in culture medium were treated with different concentrations of extract of A. pilosa. The inhibitory rate of the cells was measured by MTT assay. Cell apoptotic rate was detected by flow cytometry (FCM). Fluo-3/AM fluorescent probe was used to observe the Ca2+ variation in the tumour cells. The variation of intracellular ROS was detected by FCM. Results The extract of A. pilosa could inhibit the growth of HepG2 cells and cause apoptosis significantly. The IC50 was 127.85 μg/mL. The number of cells was decreased especially after the cells were treated 48 h by the extract of A. pilosa,and the green fluorescence was seen clearly by Fluo-3/AM fluorescent probe. The intracellular ROS was increased obviously. Conclusion The extract of A. pilosa could inhibit the growth of HepG2 cells and cause apoptosis. The releasing of the intracellular Ca2+ variation and the increasing of the intracellular ROS may be its anti-hepatocellular carcinoma mechanisms.

黑龍江省留學回國人員重點科技項目（黑人發(fā)[2009]23號）