目的 研究美洲大蠊醇提物對四氯化碳（CCl4）和刀豆蛋白A（Con A）所致小鼠肝損傷的保護(hù)作用。方法 采用ip 0.1% CCl4以及尾iv 20 mg/kg Con A造成小鼠急性肝損傷，檢測血清中ALT、AST和肝組織中SOD、MDA的水平。將肝大葉HE染色，觀察美洲大蠊醇提物對小鼠肝臟組織病理學(xué)的影響。結(jié)果 美洲大蠊醇提物能明顯降低CCl4所致的肝損傷小鼠肝臟、脾臟指數(shù)的升高，降低肝損傷小鼠血清中ALT、AST的升高（P＜0.05），降低小鼠肝組織中MDA水平、升高SOD（P＜0.05）；肝臟組織病理學(xué)觀察可見，美洲大蠊醇提物能夠減輕炎性因子的浸潤，減輕肝細(xì)胞的水腫程度，減少肝細(xì)胞壞死，促進(jìn)肝細(xì)胞的再生。結(jié)論 美洲大蠊醇提物具有顯著的抗CCl4和Con A所致肝損傷的作用。

Objective To study the protective effect of Periplaneta americana extract against liver injury in mice induced by carbon tetrachloride (CCl4) and concanavalin A (Con A). Methods Acute liver injury of mice was induced by ip injection 0.1% CCl4 or iv injection 20 mg/kg Con A. Contents of ALT, AST, SOD, and MDA were determined by UV spectrophotometer. HE staining was used to test the pathology of liver. Results P. americana extract could obviously decrease liver and spleen index of liver-injured mice caused by CCl4 and Con A, and remarkably resist the increase of ALT, AST in mice serum, MDA in hepatic tissue (P<0.05), and the decrease of SOD in hepatic tissue (P<0.05). According to the liver tissue histopathology, P. americana extract could alleviate infiltrating of the inflammatory factor, reduce the degree of liver cells′ edema and necrosis, and promote the liver cells′ regeneration.