目的 建立苦杏仁中苦杏仁酶的閃式提取工藝，并對(duì)其工藝條件進(jìn)行優(yōu)化。方法 以苦杏仁酶總活力為指標(biāo)，考察了氨水濃度、提取液用量、提取時(shí)間、中和終點(diǎn)pH值和沉淀時(shí)乙醇體積分?jǐn)?shù)對(duì)閃式提取工藝的影響；采用二硝基水楊酸（DNS）法測(cè)定苦杏仁酶的比活力，并計(jì)算其總活力。結(jié)果 苦杏仁酶的最佳提取工藝條件為：取50.0 g脫脂苦杏仁粉，使用500 mL 62.5 mmol/L預(yù)冷氨水提取120 s 后，殘?jiān)偈褂?50 mL提取液提取90 s，濾過(guò)，濾液用36%醋酸調(diào)pH 4.0，濾去雜蛋白后加入4倍量乙醇，沉淀苦杏仁酶。結(jié)論 采用閃式提取法改進(jìn)了苦杏仁酶的提取工藝，并對(duì)該工藝進(jìn)行了優(yōu)化，得出了較優(yōu)的閃式提取條件。該工藝具有周期短、操作簡(jiǎn)便、酶活力高等優(yōu)點(diǎn)，提取效果優(yōu)于傳統(tǒng)方法。

Objective To establish and optimize the extraction technology of emulsin from Armeniacae Amarum Semen using smashing tissue extraction method. Methods These factors, such as ammonia concentration, the amount of extract, extraction time, terminal point pH value of counteraction, and the volume fraction of ethanol for precipitation influencing on the extraction process of smashing tissue extraction were investigated by using total enzymatic activity of emulsin as index. The specific enzymatic activity of emulsin was determined by dinitrosalicylic acid (DNS) method, and the total enzymatic activity was calculated. Results The optimum extraction conditions were as following: 50.0 g powder of defatted Armeniacae Amarum Semen was extracted with 500 mL 0.062 5 mol/L pre-cooling ammonia for 120 s. The mixture was filtrated by gauzes and the residue was extracted with 450 mL extract for 90 s again. Collected the filtrate, adjusted its pH value to 4.0 by acetic acid, filtered hybrid protein, and precipitated the emulsin by four times the amount of ethanol. Conclusion The smashing tissue extraction is used to improve the extraction technology of emulsin from Armeniacae Amarum Semen for the first time, and the conditions of the new technology are optimized by determining the specific activity of emulsin. The results show that smashing tissue extraction has the advantages of short time, simple operation, and higher enzymatic activity, which is better than traditional extraction method.