甘草因療效顯著、藥理作用明確，一直以來都是研究的熱點。近年來有20個黃酮類化合物和6個三萜類化合物被陸續(xù)分離出來，其中包括16個新的黃酮類化合物和3個新的三萜類化合物。細胞培養(yǎng)是獲得甘草有效成分的有效途徑之一，目前應用細胞培養(yǎng)技術主要生產甘草黃酮類成分。綜述了近年來從甘草中提取的黃酮類、三萜類化合物，細胞培養(yǎng)方法對甘草黃酮類成分的影響，以及毛細管電色譜法、HPLC法、免疫測定法等檢測甘草化學成分方法的研究進展。

Glycyrrhizae Radix et Rhizoma has been the research focus because of its conspicuous healing efficacy and pharmacological activities. Recently, 20 flavonoids and 6 triterpenes compounds, including 16 new flavonoids and 3 new triterpenes have been isolated from this plant. It was also found that cell culture provided an effective way to acquire effective components of Glycyrrhizae Radix et Rhizoma. The cell culture technique is mainly used for product Glycyrrhizae Radix et Rhizoma flavonoids. In this paper, the recent progresses was reported on the chemical components of the plant, the cell culture method for the bioactive flavonoids ingredients and determination of its effective constituents using capillary electrochromatography, HPLC, and immunoassays detection as well.

“十一?五”國家科技重大專項資助項目（2009ZX09501-011）；新疆維吾爾自治區(qū)科學技術廳資助項目（200915122）