目的 建立測定青龍衣中羥基萘醌的方法，考察不同貯藏年限的青龍衣藥材中羥基萘醌的變化。方法 以胡桃醌為對照品，5%氫氧化鉀為顯色劑，在515 nm波長下，采用比色法測定青龍衣中羥基萘醌，對不同貯藏年限的青龍衣藥材中羥基萘醌進(jìn)行比較。結(jié)果 貯藏年限為一年、二年和三年的青龍衣藥材中羥基萘醌的量存在明顯差異，即隨著貯藏時(shí)間的延長，青龍衣中羥基萘醌的量逐漸下降。結(jié)論 青龍衣藥材不宜儲藏時(shí)間過長，盡量應(yīng)用當(dāng)年的藥材。

Objective To establish a determination method of hydroxyl naphthoquinones for Qinglongyi (fresh rejuvenated fruits of Juglans mandshurica), and to compare hydroxyl naphthoquinones from Qinglongyi in different storage times. Methods Juglone was used as standard, 5% KOH solution was used as chromogenic reagent, and wavelength was at 515 nm. Hydroxyl naphthoquinones in Qinglongyi in different storage times were determined by colorimetric method and compared. Results There were great differences among hydroxyl naphthoquinones contents of storage period 1, 2 and 3 years of Qinglongyi. The hydroxyl naphthoquinones contents of Qinglongyi gradually decreased with the storage time prolonged. Conclusion Qinglongyi should not be kept too long time, and should be used that very year.

國家自然科學(xué)基金資助項(xiàng)目（30600816）；高等學(xué)校博士點(diǎn)學(xué)科點(diǎn)專項(xiàng)科研基金資助項(xiàng)目（20060240001））