目的 建立同時(shí)測(cè)定糙葉五加不同藥用部位中槲皮素和山柰酚的方法。方法 采用RP-HPLC法，色譜柱為AT.Lichrom ODS-C18柱（250 mm×4.6 mm，5 μm），以甲醇–磷酸–水（50︰0.2︰49.8）為流動(dòng)相，柱溫30 ℃，體積流量1.0 mL/min，檢測(cè)波長(zhǎng)360 nm，進(jìn)樣量10 μL。結(jié)果 槲皮素在0.018～0.720 μg線性關(guān)系良好（r=0.999 6），加樣回收率為98.41%，RSD為0.91%；山柰酚在0.016～0.640 μg線性關(guān)系良好（r=0.999 7），加樣回收率為98.06%，RSD為1.39%。槲皮素和山柰酚在葉中的量最高，其次是莖，根中未檢出。結(jié)論 對(duì)糙葉五加不同藥用部位槲皮素和山柰酚進(jìn)行了測(cè)定，所建立的HPLC法穩(wěn)定性好、準(zhǔn)確性高，適用于糙葉五加中槲皮素和山柰酚的定量分析。

Objective To establish a RP-HPLC method for the determination of quercetin and kaempferol in different medicinal parts of Acanthopanax henryi. Methods The analysis was performed on an AT.Lichrom ODS-C18 column (250 mm×4.6 mm，5 μm) with methanol-phosphoric acid-water (50︰0.2︰49.8) as mobile phase．The flow rate was 1.0 mL/min．The column temperature was 30 ℃, and the detection wavelength was 360 nm. Results The established method showed good linearity for quercetin and kaempferol over the range of 0.018—0.720 μg (r=0.999 6) and 0.016—0.640 μg (r=0.999 7), respectively. The average recovery of the method for quercetin and kaempferol were 98.41% with the RSD 0.91% and 98.06% with the RSD 1.39%, respectively. The highest content of quercetin and kaempferol was in the leaves of A. henryi, followed by stems, and undetected in roots. Conclusion Contents of quercetin and kaempferol in different medicinal parts of A. henryi are determined. The established HPLC method shows good stability and high accuracy, and can be used for the quantitative analysis of quercetin and kaempferol in A. henryi.

湖南省教育廳產(chǎn)業(yè)化項(xiàng)目（09CY020）