目的 參比商品珍珠粉及珍珠蚌殼粉液相指紋特征，建立淡水養(yǎng)殖1～5年珍珠中氨基酸的液相指紋圖譜以控制質(zhì)量。方法 采用HPLC測(cè)定，色譜條件：Venusil-AA氨基酸分析柱，乙腈–0.1 mol/L的醋酸鈉水溶液（pH 6.50）為流動(dòng)相，梯度洗脫，檢測(cè)波長(zhǎng)254 nm，體積流量1.0 mL/min，柱溫45 ℃。結(jié)果 建立了HPLC指紋圖譜共有模式，得出不同品系的樣本相似度分布譜帶。結(jié)論 色譜指紋圖譜分析法能準(zhǔn)確地鑒別區(qū)分不同品系的珍珠類(lèi)產(chǎn)品，可為珍珠粉的質(zhì)量控制和真?zhèn)舞b別提供依據(jù)和方法。

Objective To set up the HPLC fingerprints of amino acids in frreshwater aquaculture pear powder for 1－5 years and compare the fingerprints with that of commercial pearl powder and of pearl shell powder so as to establish a sensitive and specific method. Methods The HPLC separation was performed on a Venusil-AA analytical column (commercial spectrum column special for analyzing amino acids) and by gradient elution with acetonitrile-0.1 mol/L sodium acetate solution (pH 6.5) at the flow rate of 1.0 mL/min. The temperature of column was 45 ℃. The detection wavelength was 254 nm. Results The common mode of HPLC fingerprints was set up and the similar degrees of commercial pearl powder and pearl shell powder to crude pearl powder were compared. Conclusion It is simple and rapid to identify and differentiate pearl powder collected from different sources with the method which can be used as a quality control item for pearl powder and its related products.