目的 對銀杏葉中總內(nèi)酯進行研究，為銀杏葉資源的合理利用提供科學(xué)依據(jù)。方法 采用HPLC-ELSD法測定不同樹齡、采收季節(jié)和干燥方法的銀杏葉中總內(nèi)酯。結(jié)果 樹齡小于10年，5～7月采收，干燥方法為曬干的銀杏葉中總內(nèi)酯含量較高。結(jié)論 銀杏葉中總內(nèi)酯的含量受樹齡、采收季節(jié)和干燥方法的影響，應(yīng)合理采收銀杏葉，保護銀杏葉資源。

Objective To determine the total lactones in Ginkgo biloba leaves and provide the scientific basis for the rational utilization of G. biloba leaves resources. Methods HPLC-ELSD method was used to determine the total lactones in G. biloba leaves at different tree ages, in different harvest seasons, and by various drying methods. Results T he highest content of the total lactone was observed in the leaves at tree age of no more than 10 years, collected from May to July, and drying under the sun. Conclusion The contents of the total lactones in G. biloba leaves are affected by tree ages, harvest seasons, and dry methods, so G. biloba leaves should be collected reasonably to protect their resources.

國家重大新藥創(chuàng)制專項（2011ZX09401-097）