目的 測定蒙古黃芪多糖中的單糖組成。方法 蒙古黃芪多糖樣品用三氟乙酸溶液水解成單糖，用1-苯基-3-甲基- 5-吡唑啉酮（PMP）衍生化，采用HPLC法于245 nm波長處檢測各單糖。結果 蒙古黃芪多糖由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、阿拉伯糖組成，物質(zhì)的量比分別為0.02∶0.05∶0.17∶1∶0.18。結論 此方法簡單、快速、重復性好，可用于蒙古黃芪多糖中單糖組成分析。

Objective To determine the monosaccharide compositions in Astragalus mongholicus var. mongholicus polysaccharides (AMMP). Methods AMMP samples were first hydrolyzed with trifluoroacetic acid, and then derivatized with 1-phenyl-3-methyl-5- pyrazolone (PMP). Monosaccharide was determined by HPLC at detective wavelength of 245 nm. Results AMMP composed of mannose, rhamonse, galacturonic acid, glucose, and arabinose with molar ratio 0.02∶0.05∶0.17∶1∶0.18. Conclusion The method is simple, fast, and reproducible, and could be used for the analysis of monosaccharide compositions in AMMP.

國家“十二五”科技支撐計劃資助項目（2011BAI07B01）