目的 觀察番茄紅素對(duì)四氯化碳（CCl4）引起的小鼠急性肝損傷的保護(hù)作用。方法 將小鼠分為正常組、模型組、番茄紅素組和陽(yáng)性藥聯(lián)苯雙酯組。番茄紅素組和聯(lián)苯雙酯組分別用番茄紅素和聯(lián)苯雙酯ig進(jìn)行預(yù)給藥干預(yù)，正常組和模型組以溶劑0.1%羧甲基纖維素鈉ig，連續(xù)給藥7 d后ip CCl4致小鼠急性肝損傷。計(jì)算肝臟指數(shù)，檢測(cè)血清中谷丙轉(zhuǎn)氨酶（ALT）、谷草轉(zhuǎn)氨酶（AST）以及肝組織中的超氧化歧化酶（SOD）、丙二醛（MDA）、乳酸脫氫酶（LDH）的水平，并觀察肝組織HE染色切片的病理變化。結(jié)果 番茄紅素能顯著降低急性肝損傷小鼠血清ALT、AST活性；升高肝組織勻漿中SOD活力，降低MDA含量和LDH活性；病理切片表明給藥組小鼠肝損傷均減輕。結(jié)論 番茄紅素對(duì)CCl4致小鼠急性肝損傷具有保護(hù)作用，其機(jī)制可能與番茄紅素所具有抗脂質(zhì)過(guò)氧化和清除體內(nèi)過(guò)多的氧自由基的作用有關(guān)。

Objective To study the protective effects of lycopene against carbon tetrachloride (CCl4)-induced acute hepatic injury in mice. Methods The mice were divided into four groups: normal, model, lycopene, and positive control (biphenyldicarboxylate) groups. The mice in lycopene and positive control groups were ig pretreated with lycopene and biphenyldicarboxylate once daily for consecutive 7 d, respectively. The mice in the normal and model groups were given 0.1% carboxymethylcellulose sodium (CMC-Na), the solvent, instead. Then, acute liver injury in mice was induced by ip injection of CCl4. After the mice were sacrificed, the hepatic indices were calculated. The activities of serum ALT and AST, hepatic SOD and LDH, and level of hepatic MDA were also determined. Histopathological changes of liver tissue slices stained by HE staining were observed under light microscope. Results The activities of ALT, AST and LDH, and level of MDA were significantly decreased by lycopene pretreatment, while activity of SOD in hepatic tissues was markedly increased. The effects of lycopene were close to those of biphenyldicarboxylate. Histopathological results also showed the obvious improvement of liver injury in lycopene pretreatment group. Conclusion Lycopene has protective effects against CCl4-induced acute hepatic injury in mice, and the mechanisms might be associated with its anti-oxidative activity.

國(guó)家自然科學(xué)基金資助項(xiàng)目（81173638，81102835，81001682）