目的 對烏拉立肽粗品純化并制備符合純度要求的烏拉立肽純品。方法 采用C18（H）ODS柱（30 mm×250 mm，10 μm）；流動相A為0.1%甲酸–水溶液，流動相B為100%乙腈；檢測波長215 nm；體積流量30 mL/min；進樣量60 mg。結(jié)果 RP-HPLC一步純化后得到烏拉立肽質(zhì)量分數(shù)達到98%。通過多次樣品收集和冷凍干燥，制備了高純度的烏拉立肽。結(jié)論 建立了烏拉立肽的純化工藝，制備出符合要求的烏拉立肽純品。

Objective To purify ularitide crude product and prepare qualified ularitide met to the purity requriement. Methods The samples were separated on C18 (H) ODS column (30 mm×250 mm, 10 μm) with 0.1% formic acid (A) - water (B) as mobile phase at a flow rate of 30 mL/min. The detection wavelegth was set at 215 nm with sample volume of 60 mg. Results The purity of ularitide could reach 98% after being purified once by RP-HPLC. The high purity ularitide was obtained after being lyophilized and collected for several times. Conclusion The purification technology is set up for ularitide and the prepared ularitide is qualified.