目的 合成一長鏈肽47肽。方法 采用Fmoc/t-Bu正交保護多肽固相合成策略，使用9-芴甲氧羰基（Fmoc）保護氨基酸的α-氨基，用Liberty微波多肽合成儀，每個耦合循環(huán)用20%哌啶脫除Fmoc保護基團，連續(xù)添加氨基酸，并用茚三酮法檢測反應終點，有效檢測反應的進度，最后用82.5%的三氟乙酸將多肽從樹脂上切割下來，得到47肽粗品。經(jīng)過反相高效液相制備系統(tǒng)純化得到多肽純品，用反相高效液相分析儀和傅里葉高分辨質譜儀驗證該產(chǎn)物。結果 高分辨質譜儀驗證了該產(chǎn)物，純品質量分數(shù)達98%。結論 微波可促進多肽特別是長鏈肽的合成。

Objective To synthesize a long-chain peptide—47 peptide. Methods Fmoc/t-Bu orthogonal protection solid phase synthesis strategy was used to synthesize the long-chain peptide, using 9-fluorenylmethoxycarbonyl (Fmoc) to protect the alpha amino of amino acid. With Liberty Microwave Peptide Synthesizer, 20% piperidine was used to remove Fmoc for each coupling cycle, amino acids were continuously added, and ninhydrin was used to detect the end point of reactions. The reaction progress was effectively detected, and finally 82.5% trifluoroacetic acid was used to cut off the peptide from resin, getting crude peptides. Finally, the crude peptide was purified by preparative RP-HPLC. The peptide was analyzed by analytical RP-HPLC and Fourier high-resolution mass spectrometer. Results The product has been validated High Resolution Mass Spectrometer and the purity of peptide is 98%. Conclusion Microwave could promote the synthesis of polypeptide, especially long-chain peptide.

國家自然科學基金資助項目（2009ZX09301-008-P-05）；國家重大新藥創(chuàng)制“十一五”科技重大專項（2011ZX09041-009）