目的 研究赤芝Ganoderma lucidum子實(shí)體中三萜酸類(lèi)成分及其抗腫瘤活性。方法 用硅膠柱色譜、反相ODS柱色譜、反相HPLC等方法分離得到13個(gè)化合物，利用波譜學(xué)方法鑒定它們的化學(xué)結(jié)構(gòu)，并進(jìn)一步確定各化合物對(duì)乳腺癌SKBR3細(xì)胞的生物活性。結(jié)果 分離得到13個(gè)三萜酸類(lèi)的化合物，分別鑒定為靈芝烯酸C（1）、靈芝酸C2（2）、靈赤酸A（3）、靈芝酸G（4）、靈芝烯酸B（5）、靈芝酸B（6）、靈芝酸H（7）、靈芝烯酸A（8）、靈芝酸I（9）、靈芝酸A（10）、靈芝烯酸K（11）、靈芝烯酸D（12）、靈芝酸D（13）?；衔?在20 μmol/L濃度下對(duì)人乳腺癌細(xì)胞SK-BR-3的抑制率為40.3%。結(jié)論 化合物11是首次從該植物中分離得到的，化合物6對(duì)人乳腺癌細(xì)胞SK-BR-3顯示了一定的抑制活性，其他化合物在該濃度下未顯示活性。

Objective To study the triterpenoid acids in sporophore of Ganoderma lucidum and their antitumor activities. Methods The compounds were isolated by the silica gel, ODS column chromatography and HPLC techniques, then their structures were identified by their physicochemical characteristics and spectral features. The inhibitory activity of all compounds against SKBR3 human breast cancer cells was assessed. Results Thirteen compounds were isolated and identified as ganoderenic acid C (1), ganoderic acid C2 (2), ganolucid acid A (3), ganoderic acid G (4), ganoderenic acid B (5), ganoderic acid B (6), ganoderic acid H (7), ganoderenic acid A (8), ganoderic acid I (9), ganoderic acid A (10), ganoderenic acid K (11), ganoderenic acid D (12), and ganoderic acid D (13). Compound 6 (20 μmol/L) exhibited an inhibitory rate of 40.3% against SKBR3 human breast cancer cells. Conclusions Compound 11 is isolated from this plant for the first time. Compound 6 exhibits the inhibition against SKBR3 human breast cancer cells at 20 μmol/L, but other compounds have no activities at the same concentration.

國(guó)家重大新藥創(chuàng)制專(zhuān)項(xiàng)（2011ZX09-401-009）