目的 研究蒙古黃芪乙醇提取物石油醚、二氯甲烷、醋酸乙酯萃取部位的化學(xué)成分。方法 利用硅膠柱色譜、凝膠柱色譜、制備液相色譜等方法進(jìn)行分離、純化，根據(jù)化合物的理化性質(zhì)和波譜數(shù)據(jù)鑒定其結(jié)構(gòu)。結(jié)果 分離得到14個(gè)化合物，分別鑒定為紅車軸草素（1）、芒柄花素（2）、芒柄花素-7-O-β-D-葡萄糖苷（3）、毛蕊異黃酮（4）、毛蕊異黃酮-7-O-β-D-葡萄糖苷（5）、(6aR,11aR)-3-羥基-9,10-二甲氧基紫檀烷（6）、(6aR,11aR)9,10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷（7）、黃芪皂苷Ι（8）、乙酰黃芪皂苷Ι（9）、黃芪皂苷ΙΙ（10）、異黃芪皂苷ΙΙ（11）、腺嘌呤（12）、β-谷甾醇（13）、胡蘿卜苷（14）。結(jié)論 此次從蒙古黃芪中主要分離得到黃酮類和皂苷類化合物。

Objective To study the chemical constituents in petroleum ether, methylene chloride, and ethyl acetate fractions in the alcohol extract from the dried roots of Astragalus membranaceus var. mongholicus. Methods The constituents were separated and purified by silica gel, Sephadex LH-20 column chromatography, and preparative HPLC. Their structures were elucidated by physicochemical properties and spectroscopic data. Results Fourteen compounds were obtained and identified as pratensein (1), formononetin (2), formononetin-7-O-β-D-glucoside (3), calycosin (4), calycosin-7-O-β-D-glucoside (5), (6aR,11aR)-3-hydroxy-9,10- dimethoxypterocarpan (6), (6aR,11aR)9,10-dimethoxypterocarpan-3-O-β-D-glucoside (7), astragaloside Ι (8), acetylastragaloside Ι (9), astragaloside ΙΙ (10), isoastragaloside ΙΙ (11), adenine (12), β-sitosterol (13), and daucosterol (14). Conclusion Flavonoids and saponins are the essential components in A. membranaceus var. mongholicus.

天津市科技創(chuàng)新體系及條件平臺建設(shè)計(jì)劃項(xiàng)目（10SYSYJC28900）；天津市高等學(xué)校科技發(fā)展基金計(jì)劃項(xiàng)目（20090223）